8d0s
From Proteopedia
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== Function == | == Function == | ||
| - | [https://www.uniprot.org/uniprot/FUT9_HUMAN FUT9_HUMAN] Catalyzes | + | [https://www.uniprot.org/uniprot/FUT9_HUMAN FUT9_HUMAN] Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, beta-D-Gal-(1->4)-beta-D-GlcNAc-) glycan attached to glycolipids and N- or O-linked glycoproteins. Fucosylates distal type 2 LacNAc and its fucosylated (H-type 2 LacNAc) and sialylated (sialyl-type 2 LacNAc) derivatives to form Lewis x (Lex) (CD15) and Lewis y (Ley) antigenic epitopes involved in cell adhesion and differentiation (PubMed:10386598, PubMed:10622713, PubMed:11278338, PubMed:12107078, PubMed:16282604, PubMed:17335083, PubMed:18395013, PubMed:23192350, PubMed:23263199, PubMed:29593094, PubMed:37202521). Generates Lex epitopes in the brain, presumably playing a role in the maintenance of neuronal stemness and neurite outgrowth in progenitor neural cells (By similarity) (PubMed:17335083, PubMed:23000574). Fucosylates the internal type 2 LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:23192350). Can also modify milk oligosaccharides, in particular type 2 tetrasaccharide LNnT (PubMed:37202521).[UniProtKB:O88819]<ref>PMID:10386598</ref> <ref>PMID:10622713</ref> <ref>PMID:11278338</ref> <ref>PMID:12107078</ref> <ref>PMID:16282604</ref> <ref>PMID:17335083</ref> <ref>PMID:18395013</ref> <ref>PMID:23000574</ref> <ref>PMID:23192350</ref> <ref>PMID:23263199</ref> <ref>PMID:29593094</ref> <ref>PMID:37202521</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mammalian cell surface and secreted glycoproteins exhibit remarkable glycan structural diversity that contributes to numerous physiological and pathogenic interactions. Terminal glycan structures include Lewis antigens synthesized by a collection of alpha1,3/4-fucosyltransferases (CAZy GT10 family). At present, the only available crystallographic structure of a GT10 member is that of the Helicobacter pylori alpha1,3-fucosyltransferase, but mammalian GT10 fucosyltransferases are distinct in sequence and substrate specificity compared with the bacterial enzyme. Here, we determined crystal structures of human FUT9, an alpha1,3-fucosyltransferase that generates Lewis(x) and Lewis(y) antigens, in complex with GDP, acceptor glycans, and as a FUT9-donor analog-acceptor Michaelis complex. The structures reveal substrate specificity determinants and allow prediction of a catalytic model supported by kinetic analyses of numerous active site mutants. Comparisons with other GT10 fucosyltransferases and GT-B fold glycosyltransferases provide evidence for modular evolution of donor- and acceptor-binding sites and specificity for Lewis antigen synthesis among mammalian GT10 fucosyltransferases. | ||
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| + | Structural basis for Lewis antigen synthesis by the alpha1,3-fucosyltransferase FUT9.,Kadirvelraj R, Boruah BM, Wang S, Chapla D, Huang C, Ramiah A, Hudson KL, Prudden AR, Boons GJ, Withers SG, Wood ZA, Moremen KW Nat Chem Biol. 2023 Aug;19(8):1022-1030. doi: 10.1038/s41589-023-01345-y. Epub , 2023 May 18. PMID:37202521<ref>PMID:37202521</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8d0s" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
Human FUT9 bound to GDP and LNnT
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