1u8y
From Proteopedia
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'''CRystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap''' | '''CRystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap''' | ||
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[[Category: Nicely, N I.]] | [[Category: Nicely, N I.]] | ||
[[Category: Serrano, V de.]] | [[Category: Serrano, V de.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:54:44 2008'' | |
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Revision as of 07:54, 3 May 2008
CRystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap
Overview
RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap.
About this Structure
1U8Y is a Single protein structure of sequence from Saguinus oedipus. Full crystallographic information is available from OCA.
Reference
Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:15530367 Page seeded by OCA on Sat May 3 10:54:44 2008
