1ucy

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[[Image:1ucy.jpg|left|200px]]
[[Image:1ucy.jpg|left|200px]]
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{{Structure
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|PDB= 1ucy |SIZE=350|CAPTION= <scene name='initialview01'>1ucy</scene>, resolution 2.2&Aring;
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The line below this paragraph, containing "STRUCTURE_1ucy", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=OPR:C-(3-OXOPROPYL)ARGININE'>OPR</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span>
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{{STRUCTURE_1ucy| PDB=1ucy | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucy OCA], [http://www.ebi.ac.uk/pdbsum/1ucy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ucy RCSB]</span>
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'''THROMBIN COMPLEXED WITH FIBRINOPEPTIDE A ALPHA (RESIDUES 7-19). THREE COMPLEXES, ONE WITH EPSILON-THROMBIN AND TWO WITH ALPHA-THROMBIN'''
'''THROMBIN COMPLEXED WITH FIBRINOPEPTIDE A ALPHA (RESIDUES 7-19). THREE COMPLEXES, ONE WITH EPSILON-THROMBIN AND TWO WITH ALPHA-THROMBIN'''
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[[Category: Edwards, B.]]
[[Category: Edwards, B.]]
[[Category: Martin, P.]]
[[Category: Martin, P.]]
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[[Category: complex (serine protease/coagulation)]]
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[[Category: Protease]]
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[[Category: protease]]
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[[Category: Serine]]
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[[Category: serine]]
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[[Category: Thrombin]]
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[[Category: thrombin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:03:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:08:41 2008''
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Revision as of 08:03, 3 May 2008

Image:1ucy.jpg

Template:STRUCTURE 1ucy

THROMBIN COMPLEXED WITH FIBRINOPEPTIDE A ALPHA (RESIDUES 7-19). THREE COMPLEXES, ONE WITH EPSILON-THROMBIN AND TWO WITH ALPHA-THROMBIN


Overview

The crystal structure of the noncovalent complex of bovine thrombin and a fibrinogen-A alpha tridecapeptide substrate analog, G17 psi, in which the scissile bond amide nitrogen of Gly-17f has been replaced by a methylene carbon, has been determined at 2.3 A resolution with an R factor of 17.1%. The geometry of the active site indicates that the crystal structure is a close model of the true Michaelis complex. The three independently determined thrombin/G17 psi complexes in the crystal asymmetric unit reveal novel interactions for the P2' and P3' residues-Pro-18f and Arg-19f, respectively-on the carboxyl-terminal side of the scissile bond and confirm previously observed interactions of the P1 (Arg-16f) through P10 (Asp-7f) positions on the amino-terminal side. The thrombin S2' binding site for Pro-18f, as observed in all three complexes, differs from that predicted by modeling studies and is notable for including two carbonyl oxygens of the thrombin main chain. Arg-19f occupies two binding sites on thrombin, S3'A and S3'B, which have dramatically different placements for the arginyl side chain and carboxyl terminus.

About this Structure

1UCY is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

Bovine thrombin complexed with an uncleavable analog of residues 7-19 of fibrinogen A alpha: geometry of the catalytic triad and interactions of the P1', P2', and P3' substrate residues., Martin PD, Malkowski MG, DiMaio J, Konishi Y, Ni F, Edwards BF, Biochemistry. 1996 Oct 8;35(40):13030-9. PMID:8855938 Page seeded by OCA on Sat May 3 11:03:19 2008

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OCA

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