2oto

From Proteopedia

(Difference between revisions)

Current revision

N-terminal fragment of Streptococcus pyogenes M1 protein

Structural highlights

2oto is a 4 chain structure with sequence from Streptococcus pyogenes serotype M1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.04Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Antigenically variable M proteins are major virulence factors and immunogens of the human pathogen group A Streptococcus (GAS). Here, we report the approximately 3 angstrom resolution structure of a GAS M1 fragment containing the regions responsible for eliciting type-specific, protective immunity and for binding fibrinogen, which promotes M1 proinflammatory and antiphagocytic functions. The structure revealed substantial irregularities and instabilities throughout the coiled coil of the M1 fragment. Similar structural irregularities occur in myosin and tropomyosin, explaining the patterns of cross-reactivity seen in autoimmune sequelae of GAS infection. Sequence idealization of a large segment of the M1 coiled coil enhanced stability but diminished fibrinogen binding, proinflammatory effects, and antibody cross-reactivity, whereas it left protective immunogenicity undiminished. Idealized M proteins appear to have promise as vaccine immunogens.

Coiled-coil irregularities and instabilities in group A Streptococcus M1 are required for virulence.,McNamara C, Zinkernagel AS, Macheboeuf P, Cunningham MW, Nizet V, Ghosh P Science. 2008 Mar 7;319(5868):1405-8. PMID:18323455^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ McNamara C, Zinkernagel AS, Macheboeuf P, Cunningham MW, Nizet V, Ghosh P. Coiled-coil irregularities and instabilities in group A Streptococcus M1 are required for virulence. Science. 2008 Mar 7;319(5868):1405-8. PMID:18323455 doi:http://dx.doi.org/319/5868/1405

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2oto"

Categories: Large Structures | Streptococcus pyogenes serotype M1 | Ghosh P | McNamara CW

@@ Line 3: / Line 3: @@
 <StructureSection load='2oto' size='340' side='right'caption='[[2oto]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2oto]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Strp1 Strp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OTO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2oto]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OTO FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.04&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">emm1.0 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301447 STRP1])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oto OCA], [https://pdbe.org/2oto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oto RCSB], [https://www.ebi.ac.uk/pdbsum/2oto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oto ProSAT]</span></td></tr>
 </table>
@@ Line 13: / Line 13: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/2oto_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 32: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Strp1]]
+[[Category: Streptococcus pyogenes serotype M1]]
-[[Category: Ghosh, P]]
+[[Category: Ghosh P]]
-[[Category: McNamara, C W]]
+[[Category: McNamara CW]]
-[[Category: Fibrinogen-binding]]
-[[Category: Helical coiled coil]]
-[[Category: Surface active protein]]
-[[Category: Toxin]]
-[[Category: Virulence factor]]

2oto

From Proteopedia

Current revision

N-terminal fragment of Streptococcus pyogenes M1 protein

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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