1ue8
From Proteopedia
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'''Crystal Structure of Thermophilic Cytochrome P450 from Sulfolobus tokodaii''' | '''Crystal Structure of Thermophilic Cytochrome P450 from Sulfolobus tokodaii''' | ||
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[[Category: Nakamura, N.]] | [[Category: Nakamura, N.]] | ||
[[Category: Ohno, H.]] | [[Category: Ohno, H.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:06:01 2008'' | |
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Revision as of 08:06, 3 May 2008
Crystal Structure of Thermophilic Cytochrome P450 from Sulfolobus tokodaii
Overview
Cytochrome P450 from thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7 (P450st) has been expressed in Escherichia coli and purified at high homogeneity. P450st was crystallized in an orthorhombic system with the space group P2(1)2(1)2(1) and cell dimensions of a=53.6 A, b=55.1 A, and c=130.9 A, and the structure was determined at a 3.0 A resolution. The final R-factor was 0.194 (Rfree=0.235). Structural comparison with cytochrome P450 from S. solfataricus (CYP119) suggests that the region composed of the F to G helices and the Cl- binding site is responsible for the affinity for a ligand coordinating heme iron. Direct electrochemistry of P450st in a didodecyldimethylammonium bromide (DDAB) film on a plastic formed carbon (PFC) electrode has also been demonstrated. A quasi-reversible redox response has been observed even at elevated temperatures of up to 80 degrees C.
About this Structure
1UE8 is a Single protein structure of sequence from Sulfolobus tokodaii. Full crystallographic information is available from OCA.
Reference
Structure and direct electrochemistry of cytochrome P450 from the thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7., Oku Y, Ohtaki A, Kamitori S, Nakamura N, Yohda M, Ohno H, Kawarabayasi Y, J Inorg Biochem. 2004 Jul;98(7):1194-9. PMID:15219985 Page seeded by OCA on Sat May 3 11:06:01 2008
