Structural highlights
Function
CUTC_HUMAN May play a role in copper homeostasis. Can bind one Cu(1+) per subunit.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Copper is an essential trace element to life and particularly plays a pivotal role in the physiology of aerobic organisms. The Cut protein family is associated with copper homeostasis and involved in uptake, storage, delivery, and efflux of copper. CutC is a member of the Cut family and is suggested to be involved in efflux trafficking of cuprous ion. We report here the biochemical and structural characterization of human CutC (hCutC). hCutC can bind Cu(I) with a stoichiometry of 1:1 and an apparent dissociation constant of 15.5+/-2.8 microM. hCutC assumes a typical TIM-barrel fold and forms a tetramer in both crystal structure and solution which is different from the dimeric architecture of the bacterial CutC. Structure analysis and sequence comparison of CutC proteins from different species reveal two strictly conserved Cys residues on the inner surface of the C-terminal end of the TIM-barrel. Mutations of the two Cys residues can significantly impair the binding ability of hCutC with Cu(I). Our results suggest that hCutC functions as an enzyme with Cu(I) as a cofactor rather than a copper transporter and the potential Cu(I)-binding site consists of the two Cys residues and other conserved residues in the vicinity.
Crystal structure of human copper homeostasis protein CutC reveals a potential copper-binding site.,Li Y, Du J, Zhang P, Ding J J Struct Biol. 2010 Mar;169(3):399-405. doi: 10.1016/j.jsb.2009.10.012. Epub 2009, Oct 28. PMID:19878721[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Li J, Ji C, Chen J, Yang Z, Wang Y, Fei X, Zheng M, Gu X, Wen G, Xie Y, Mao Y. Identification and characterization of a novel Cut family cDNA that encodes human copper transporter protein CutC. Biochem Biophys Res Commun. 2005 Nov 11;337(1):179-83. PMID:16182249 doi:http://dx.doi.org/10.1016/j.bbrc.2005.09.029
- ↑ Li Y, Du J, Zhang P, Ding J. Crystal structure of human copper homeostasis protein CutC reveals a potential copper-binding site. J Struct Biol. 2010 Mar;169(3):399-405. doi: 10.1016/j.jsb.2009.10.012. Epub 2009, Oct 28. PMID:19878721 doi:http://dx.doi.org/10.1016/j.jsb.2009.10.012
- ↑ Li Y, Du J, Zhang P, Ding J. Crystal structure of human copper homeostasis protein CutC reveals a potential copper-binding site. J Struct Biol. 2010 Mar;169(3):399-405. doi: 10.1016/j.jsb.2009.10.012. Epub 2009, Oct 28. PMID:19878721 doi:http://dx.doi.org/10.1016/j.jsb.2009.10.012
