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| | <StructureSection load='3ust' size='340' side='right'caption='[[3ust]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='3ust' size='340' side='right'caption='[[3ust]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ust]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bmnpv Bmnpv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UST FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ust]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_nucleopolyhedrovirus Bombyx mori nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UST FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Orf_75 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271108 BmNPV])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ust FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ust OCA], [https://pdbe.org/3ust PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ust RCSB], [https://www.ebi.ac.uk/pdbsum/3ust PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ust ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ust FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ust OCA], [https://pdbe.org/3ust PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ust RCSB], [https://www.ebi.ac.uk/pdbsum/3ust PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ust ProSAT]</span></td></tr> |
| | </table> | | </table> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bmnpv]] | + | [[Category: Bombyx mori nucleopolyhedrovirus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hou, Y]] | + | [[Category: Hou Y]] |
| - | [[Category: Xia, Q]] | + | [[Category: Xia Q]] |
| - | [[Category: Yuan, Y A]] | + | [[Category: Yuan YA]] |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Sulfhydryl oxidase]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Bombyx mori nucleopolyhedrovirus (BmNPV) triggers the global shutdown of host silkworm gene expression and protein synthesis approximately 12-18 h post-infection. Genome sequence analysis suggests that BmNPV ORF75 could be a flavin adenine dinucleotide (FAD)-linked thiol oxidase essential for virion assembly and virus propagation. Here, we report the crystal structure of BmNPV ORF75 at 2.1 A (0.21 nm). The structure of BmNPV ORF75 resembles that of the thiol oxidase domain of human quiescin thiol oxidase (QSOX), displaying a pseudo-dimer of canonical and non-canonical thiol oxidase domains. However, BmNPV ORF75 is further dimerized by its C-terminal canonical thiol oxidase domain. Within the unique quaternary structural arrangement, the FAD-binding pocket and the characteristic CXXC motif from each monomer is 35 A (3.5 nm) away from that of its corresponding molecule, which suggests that BmNPV ORF75 might adopt a deviant mechanism from that of QSOX to catalyse disulfide bond formation. Our thiol oxidase activity assay on the point mutations of the conserved residues participating in FAD recognition reveals an aromatic cage next to the FAD isoalloxazine moiety for substrate binding. These data suggest that the thiol oxidase activity of BmNPV ORF75 could be critical to catalyse the formation of the disulfide bonds of certain BmNPV proteins essential for BmNPV virion assembly.
Crystal structure of Bombyx mori nucleopolyhedrovirus ORF75 reveals a pseudo-dimer of thiol oxidase domains with a putative substrate-binding pocket.,Hou Y, Xia Q, Yuan YA J Gen Virol. 2012 Oct;93(Pt 10):2142-51. Epub 2012 Jul 4. PMID:22764321[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hou Y, Xia Q, Yuan YA. Crystal structure of Bombyx mori nucleopolyhedrovirus ORF75 reveals a pseudo-dimer of thiol oxidase domains with a putative substrate-binding pocket. J Gen Virol. 2012 Oct;93(Pt 10):2142-51. Epub 2012 Jul 4. PMID:22764321 doi:http://dx.doi.org/10.1099/vir.0.042747-0
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