|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6cc2' size='340' side='right'caption='[[6cc2]], [[Resolution|resolution]] 1.66Å' scene=''> | | <StructureSection load='6cc2' size='340' side='right'caption='[[6cc2]], [[Resolution|resolution]] 1.66Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cc2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CC2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CC2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CL6EHI_049900 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cc2 OCA], [https://pdbe.org/6cc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cc2 RCSB], [https://www.ebi.ac.uk/pdbsum/6cc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cc2 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cc2 OCA], [http://pdbe.org/6cc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cc2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cc2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cc2 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C4LUI8_ENTH1 C4LUI8_ENTH1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Enthi]] | + | [[Category: Entamoeba histolytica]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Aihara, H]] | + | [[Category: Aihara H]] |
| - | [[Category: Bielinsky, A]] | + | [[Category: Bielinsky A]] |
| - | [[Category: Kurahashi, K]] | + | [[Category: Kurahashi K]] |
| - | [[Category: Kurniawan, F]] | + | [[Category: Kurniawan F]] |
| - | [[Category: Shi, K]] | + | [[Category: Shi K]] |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Cell division control protein]]
| + | |
| Structural highlights
6cc2 is a 1 chain structure with sequence from Entamoeba histolytica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.66Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
C4LUI8_ENTH1
Publication Abstract from PubMed
Cdc45 plays a critical role at the core of the eukaryotic DNA replisome, serving as an essential scaffolding component of the replicative helicase holoenzyme Cdc45-MCM-GINS (CMG) complex. A 1.66-A-resolution crystal structure of the full-length Cdc45 protein from Entamoeba histolytica shows a protein fold similar to that observed previously for human Cdc45 in its active conformation, featuring the overall disk-like monomer shape and intimate contacts between the N- and C-terminal DHH domains. However, the E. histolytica Cdc45 structure shows several unique features, including a distinct orientation of the C-terminal DHHA1 domain, concomitant disordering of the adjacent protruding alpha-helical segment implicated in DNA polymerase epsilon interactions, and a unique conformation of the GINS/Mcm5-binding loop. These structural observations collectively suggest the possibility that Cdc45 can sample multiple conformations corresponding to different functional states. We propose that such conformational switch of Cdc45 may allow regulation of protein-protein interactions important in DNA replication.
Crystal Structure of Entamoeba histolytica Cdc45 Suggests a Conformational Switch that May Regulate DNA Replication.,Kurniawan F, Shi K, Kurahashi K, Bielinsky AK, Aihara H iScience. 2018 May 25;3:102-109. doi: 10.1016/j.isci.2018.04.011. PMID:29901028[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kurniawan F, Shi K, Kurahashi K, Bielinsky AK, Aihara H. Crystal Structure of Entamoeba histolytica Cdc45 Suggests a Conformational Switch that May Regulate DNA Replication. iScience. 2018 May 25;3:102-109. doi: 10.1016/j.isci.2018.04.011. PMID:29901028 doi:http://dx.doi.org/10.1016/j.isci.2018.04.011
|
