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| | <StructureSection load='6d9w' size='340' side='right'caption='[[6d9w]], [[Resolution|resolution]] 3.94Å' scene=''> | | <StructureSection load='6d9w' size='340' side='right'caption='[[6d9w]], [[Resolution|resolution]] 3.94Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6d9w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Deira Deira] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D9W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6d9w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D9W FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OS:OSMIUM+ION'>OS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.941Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5kte|5kte]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OS:OSMIUM+ION'>OS</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mntH, DR_1709 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243230 DEIRA])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d9w OCA], [https://pdbe.org/6d9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d9w RCSB], [https://www.ebi.ac.uk/pdbsum/6d9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d9w ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d9w OCA], [http://pdbe.org/6d9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d9w RCSB], [http://www.ebi.ac.uk/pdbsum/6d9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d9w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MNTH_DEIRA MNTH_DEIRA]] H(+)-stimulated, divalent metal cation uptake system. | + | [https://www.uniprot.org/uniprot/MNTH_DEIRA MNTH_DEIRA] H(+)-stimulated, divalent metal cation uptake system. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Deira]] | + | [[Category: Deinococcus radiodurans R1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Bane, L B]] | + | [[Category: Bane LB]] |
| - | [[Category: Bozzi, A T]] | + | [[Category: Bozzi AT]] |
| - | [[Category: Gaudet, R]] | + | [[Category: Gaudet R]] |
| - | [[Category: Singharoy, A]] | + | [[Category: Singharoy A]] |
| - | [[Category: Weihofen, W A]] | + | [[Category: Weihofen WA]] |
| - | [[Category: Zimanyi, C M]] | + | [[Category: Zimanyi CM]] |
| - | [[Category: Leut-fold]]
| + | |
| - | [[Category: Proton]]
| + | |
| - | [[Category: Secondary transporter]]
| + | |
| - | [[Category: Transition metal]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
MNTH_DEIRA H(+)-stimulated, divalent metal cation uptake system.
Publication Abstract from PubMed
Nramp family transporters-expressed in organisms from bacteria to humans-enable uptake of essential divalent transition metals via an alternating-access mechanism that also involves proton transport. We present high-resolution structures of Deinococcus radiodurans (Dra)Nramp in multiple conformations to provide a thorough description of the Nramp transport cycle by identifying the key intramolecular rearrangements and changes to the metal coordination sphere. Strikingly, while metal transport requires cycling from outward- to inward-open states, efficient proton transport still occurs in outward-locked (but not inward-locked) DraNramp. We propose a model in which metal and proton enter the transporter via the same external pathway to the binding site, but follow separate routes to the cytoplasm, which could facilitate the co-transport of two cationic species. Our results illustrate the flexibility of the LeuT fold to support a broad range of substrate transport and conformational change mechanisms.
Structures in multiple conformations reveal distinct transition metal and proton pathways in an Nramp transporter.,Bozzi AT, Zimanyi CM, Nicoludis JM, Lee BK, Zhang CH, Gaudet R Elife. 2019 Feb 4;8. pii: 41124. doi: 10.7554/eLife.41124. PMID:30714568[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bozzi AT, Zimanyi CM, Nicoludis JM, Lee BK, Zhang CH, Gaudet R. Structures in multiple conformations reveal distinct transition metal and proton pathways in an Nramp transporter. Elife. 2019 Feb 4;8. pii: 41124. doi: 10.7554/eLife.41124. PMID:30714568 doi:http://dx.doi.org/10.7554/eLife.41124
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