6e63

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody TB31F

Structural highlights

6e63 is a 6 chain structure with sequence from Homo sapiens and Plasmodium falciparum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P4548_PLAF7 Gametocyte surface protein required for male fertility.

Publication Abstract from PubMed

Interventions that can block the transmission of malaria-causing Plasmodium falciparum (Pf) between the human host and Anopheles vector have the potential to reduce the incidence of malaria. Pfs48/45 is a gametocyte surface protein critical for parasite development and transmission, and its targeting by monoclonal antibody (mAb) 85RF45.1 leads to the potent reduction of parasite transmission. Here, we reveal how the Pfs48/45 6C domain adopts a (SAG1)-related-sequence (SRS) fold. We structurally delineate potent epitope I and show how mAb 85RF45.1 recognizes an electronegative surface with nanomolar affinity. Analysis of Pfs48/45 sequences reveals that polymorphisms are rare for residues involved at the binding interface. Humanization of rat-derived mAb 85RF45.1 conserved the mode of recognition and activity of the parental antibody, while also improving its thermostability. Our work has implications for the development of transmission-blocking interventions, both through improving vaccine designs and the testing of passive delivery of mAbs in humans.

Structural delineation of potent transmission-blocking epitope I on malaria antigen Pfs48/45.,Kundu P, Semesi A, Jore MM, Morin MJ, Price VL, Liang A, Li J, Miura K, Sauerwein RW, King CR, Julien JP Nat Commun. 2018 Oct 26;9(1):4458. doi: 10.1038/s41467-018-06742-9. PMID:30367064^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kundu P, Semesi A, Jore MM, Morin MJ, Price VL, Liang A, Li J, Miura K, Sauerwein RW, King CR, Julien JP. Structural delineation of potent transmission-blocking epitope I on malaria antigen Pfs48/45. Nat Commun. 2018 Oct 26;9(1):4458. doi: 10.1038/s41467-018-06742-9. PMID:30367064 doi:http://dx.doi.org/10.1038/s41467-018-06742-9

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6e63"

@@ Line 3: / Line 3: @@
 <StructureSection load='6e63' size='340' side='right'caption='[[6e63]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6e63]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E63 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6E63 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6e63]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E63 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6e63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e63 OCA], [http://pdbe.org/6e63 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e63 RCSB], [http://www.ebi.ac.uk/pdbsum/6e63 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e63 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e63 OCA], [https://pdbe.org/6e63 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e63 RCSB], [https://www.ebi.ac.uk/pdbsum/6e63 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e63 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/P4548_PLAF7 P4548_PLAF7] Gametocyte surface protein required for male fertility.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 19: @@
 </div>
 <div class="pdbe-citations 6e63" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Plafa]]
+[[Category: Plasmodium falciparum]]
-[[Category: Julien, J P]]
+[[Category: Julien JP]]
-[[Category: Kundu, P]]
+[[Category: Kundu P]]
-[[Category: Semesi, A]]
+[[Category: Semesi A]]
-[[Category: Antibody]]
-[[Category: Antigen]]
-[[Category: Immune system]]
-[[Category: Malaria]]
-[[Category: Transmission-blocking]]

6e63

From Proteopedia

Current revision

Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody TB31F

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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