7byf
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==The crystal structure of mouse ORF10-Rae1-Nup98 complex== |
| - | <StructureSection load='7byf' size='340' side='right'caption='[[7byf]]' scene=''> | + | <StructureSection load='7byf' size='340' side='right'caption='[[7byf]], [[Resolution|resolution]] 2.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7byf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Murid_gammaherpesvirus_4 Murid gammaherpesvirus 4] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BYF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BYF FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7byf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7byf OCA], [https://pdbe.org/7byf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7byf RCSB], [https://www.ebi.ac.uk/pdbsum/7byf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7byf ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7byf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7byf OCA], [https://pdbe.org/7byf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7byf RCSB], [https://www.ebi.ac.uk/pdbsum/7byf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7byf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RAE1L_MOUSE RAE1L_MOUSE] Plays a role in mitotic bipolar spindle formation. Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.[UniProtKB:P78406] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Viruses employ multiple strategies to inhibit host mRNA nuclear export. Distinct to the generally nonselective inhibition mechanisms, ORF10 from gammaherpesviruses inhibits mRNA export in a transcript-selective manner by interacting with Rae1 (RNA export 1) and Nup98 (nucleoporin 98). We now report the structure of ORF10 from MHV-68 (murine gammaherpesvirus 68) bound to the Rae1-Nup98 heterodimer, thereby revealing detailed intermolecular interactions. Structural and functional assays highlight that two highly conserved residues of ORF10, L60 and M413, play critical roles in both complex assembly and mRNA export inhibition. Interestingly, although ORF10 occupies the RNA-binding groove of Rae1-Nup98, the ORF10-Rae1-Nup98 ternary complex still maintains a comparable RNA-binding ability due to the ORF10-RNA direct interaction. Moreover, mutations on the RNA-binding surface of ORF10 disrupt its function of mRNA export inhibition. Our work demonstrates the molecular mechanism of ORF10-mediated selective inhibition and provides insights into the functions of Rae1-Nup98 in regulating host mRNA export. | ||
| + | |||
| + | Molecular mechanism underlying selective inhibition of mRNA nuclear export by herpesvirus protein ORF10.,Feng H, Tian H, Wang Y, Zhang Q, Lin N, Liu S, Yu Y, Deng H, Gao P Proc Natl Acad Sci U S A. 2020 Oct 27;117(43):26719-26727. doi: , 10.1073/pnas.2007774117. Epub 2020 Oct 8. PMID:33033226<ref>PMID:33033226</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7byf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Murid gammaherpesvirus 4]] |
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Feng H]] | ||
| + | [[Category: Gao P]] | ||
Current revision
The crystal structure of mouse ORF10-Rae1-Nup98 complex
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