7l2z
From Proteopedia
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==Bacterial cellulose synthase BcsB hexamer== | ==Bacterial cellulose synthase BcsB hexamer== | ||
| - | <StructureSection load='7l2z' size='340' side='right'caption='[[7l2z]]' scene=''> | + | <StructureSection load='7l2z' size='340' side='right'caption='[[7l2z]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L2Z FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L2Z FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l2z OCA], [https://pdbe.org/7l2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l2z RCSB], [https://www.ebi.ac.uk/pdbsum/7l2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l2z ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l2z OCA], [https://pdbe.org/7l2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l2z RCSB], [https://www.ebi.ac.uk/pdbsum/7l2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l2z ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cellulose is frequently found in communities of sessile bacteria called biofilms. Escherichia coli and other enterobacteriaceae modify cellulose with phosphoethanolamine (pEtN) to promote host tissue adhesion. The E. coli pEtN cellulose biosynthesis machinery contains the catalytic BcsA-B complex that synthesizes and secretes cellulose, in addition to five other subunits. The membrane-anchored periplasmic BcsG subunit catalyzes pEtN modification. Here we present the structure of the roughly 1 MDa E. coli Bcs complex, consisting of one BcsA enzyme associated with six copies of BcsB, determined by single-particle cryo-electron microscopy. BcsB homo-oligomerizes primarily through interactions between its carbohydrate-binding domains as well as intermolecular beta-sheet formation. The BcsB hexamer creates a half spiral whose open side accommodates two BcsG subunits, directly adjacent to BcsA's periplasmic channel exit. The cytosolic BcsE and BcsQ subunits associate with BcsA's regulatory PilZ domain. The macrocomplex is a fascinating example of cellulose synthase specification. | ||
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| + | Molecular organization of the E. coli cellulose synthase macrocomplex.,Acheson JF, Ho R, Goularte NF, Cegelski L, Zimmer J Nat Struct Mol Biol. 2021 Mar;28(3):310-318. doi: 10.1038/s41594-021-00569-7., Epub 2021 Mar 11. PMID:33712813<ref>PMID:33712813</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7l2z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Bacterial cellulose synthase BcsB hexamer
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