7p5w
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb2== |
| - | <StructureSection load='7p5w' size='340' side='right'caption='[[7p5w]]' scene=''> | + | <StructureSection load='7p5w' size='340' side='right'caption='[[7p5w]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7p5w]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P5W FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p5w OCA], [https://pdbe.org/7p5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p5w RCSB], [https://www.ebi.ac.uk/pdbsum/7p5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p5w ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p5w OCA], [https://pdbe.org/7p5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p5w RCSB], [https://www.ebi.ac.uk/pdbsum/7p5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p5w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LRC8A_MOUSE LRC8A_MOUSE] Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. Required for channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition. Can form functional channels by itself (in vitro) (By similarity). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746, PubMed:24752297). Also required for T-cell development (PubMed:24752297).[UniProtKB:Q8IWT6]<ref>PMID:14660746</ref> <ref>PMID:24752297</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Members of the LRRC8 family form heteromeric assemblies, which function as volume-regulated anion channels. These modular proteins consist of a transmembrane pore and cytoplasmic leucine-rich repeat (LRR) domains. Despite their known molecular architecture, the mechanism of activation and the role of the LRR domains in this process has remained elusive. Here we address this question by generating synthetic nanobodies, termed sybodies, which target the LRR domain of the obligatory subunit LRRC8A. We use these binders to investigate their interaction with homomeric LRRC8A channels by cryo-electron microscopy and the consequent effect on channel activation by electrophysiology. The five identified sybodies either inhibit or enhance activity by binding to distinct epitopes of the LRR domain, thereby altering channel conformations. In combination, our work provides a set of specific modulators of LRRC8 proteins and reveals the role of their cytoplasmic domains as regulators of channel activity by allosteric mechanisms. | ||
| + | |||
| + | Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains.,Deneka D, Rutz S, Hutter CAJ, Seeger MA, Sawicka M, Dutzler R Nat Commun. 2021 Sep 14;12(1):5435. doi: 10.1038/s41467-021-25742-w. PMID:34521847<ref>PMID:34521847</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7p5w" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Ion channels 3D structures|Ion channels 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mus musculus]] |
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Deneka D]] | ||
| + | [[Category: Rutz S]] | ||
| + | [[Category: Sawicka M]] | ||
Current revision
Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb2
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