8d97
From Proteopedia
(Difference between revisions)
| Line 4: | Line 4: | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8d97]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D97 FirstGlance]. <br> | <table><tr><td colspan='2'>[[8d97]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D97 FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d97 OCA], [https://pdbe.org/8d97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d97 RCSB], [https://www.ebi.ac.uk/pdbsum/8d97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d97 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d97 OCA], [https://pdbe.org/8d97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d97 RCSB], [https://www.ebi.ac.uk/pdbsum/8d97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d97 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| Line 11: | Line 12: | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| - | The | + | The CRISPR-Cas type III-E RNA-targeting effector complex gRAMP/Cas7-11 is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here, we use cryo-electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms. Target-guide pairing extending into the 5' region of the guide RNA displaces a gating loop in gRAMP, which triggers an extensive conformational relay that allosterically aligns the protease catalytic dyad and opens an amino acid side-chain-binding pocket. We further define Csx30 as the endogenous protein substrate that is site-specifically proteolyzed by RNA-activated Craspase. This protease activity is switched off by target RNA cleavage by gRAMP and is not activated by RNA targets containing a matching protospacer flanking sequence. We thus conclude that Craspase is a target RNA-activated protease with self-regulatory capacity. |
| - | Craspase is a CRISPR RNA-guided, RNA-activated protease.,Hu C, van Beljouw SPB, Nam KH, Schuler G, Ding F, Cui Y, Rodriguez-Molina A, Haagsma AC, Valk M, Pabst M, Brouns SJJ, Ke A Science. 2022 | + | Craspase is a CRISPR RNA-guided, RNA-activated protease.,Hu C, van Beljouw SPB, Nam KH, Schuler G, Ding F, Cui Y, Rodriguez-Molina A, Haagsma AC, Valk M, Pabst M, Brouns SJJ, Ke A Science. 2022 Sep 16;377(6612):1278-1285. doi: 10.1126/science.add5064. Epub 2022 , Aug 25. PMID:36007061<ref>PMID:36007061</ref> |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
Current revision
Apo gRAMP
| |||||||||||
