1um2
From Proteopedia
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[[Image:1um2.jpg|left|200px]] | [[Image:1um2.jpg|left|200px]] | ||
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'''Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment''' | '''Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment''' | ||
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==Reference== | ==Reference== | ||
Protein splicing of yeast VMA1-derived endonuclease via thiazolidine intermediates., Mizutani R, Anraku Y, Satow Y, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):109-12. Epub 2003 Nov 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14646148 14646148] | Protein splicing of yeast VMA1-derived endonuclease via thiazolidine intermediates., Mizutani R, Anraku Y, Satow Y, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):109-12. Epub 2003 Nov 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14646148 14646148] | ||
| - | [[Category: H(+)-transporting two-sector ATPase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: Mizutani, R.]] | [[Category: Mizutani, R.]] | ||
[[Category: Satow, Y.]] | [[Category: Satow, Y.]] | ||
| - | [[Category: | + | [[Category: Extein]] |
| - | [[Category: | + | [[Category: Intein]] |
| - | [[Category: | + | [[Category: Protein splicing]] |
| - | [[Category: | + | [[Category: Thiazolidine]] |
| - | [[Category: | + | [[Category: Vde]] |
| - | [[Category: | + | [[Category: Vma1-derived endonuclease]] |
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Revision as of 08:24, 3 May 2008
Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment
Overview
Protein splicing precisely excises out an internal intein segment from a protein precursor, and concomitantly ligates the N- and C-terminal extein polypeptides flanking the intein. A recombinant X10SNS bearing N- and C-extein polypeptides has been prepared for the intein endonuclease derived from the Saccharomyces cerevisiae VMA1 gene. X10SNS has replacements of C284S, H362N and C738S, and forms the intein and extein segments in the crystal lattice. The crystal structure of X10SNS revealed a linkage between the N- and C-extein segments, and showed that the C284 amino group of the resultant intein segment is in interaction with the G283 O atom of the N-extein segment. A mechanism for the final S --> N acyl shift step proposes that a tetrahedral intermediate involves a five-membered thiazolidine ring at G283-C738 junction. An oxyanion of the thiazolidine intermediate is to be stabilized by the C284 N atom.
About this Structure
1UM2 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Protein splicing of yeast VMA1-derived endonuclease via thiazolidine intermediates., Mizutani R, Anraku Y, Satow Y, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):109-12. Epub 2003 Nov 28. PMID:14646148 Page seeded by OCA on Sat May 3 11:24:45 2008
