1umu
From Proteopedia
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'''STRUCTURE DETERMINATION OF UMUD' BY MAD PHASING OF THE SELENOMETHIONYL PROTEIN''' | '''STRUCTURE DETERMINATION OF UMUD' BY MAD PHASING OF THE SELENOMETHIONYL PROTEIN''' | ||
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[[Category: Hendrickson, W A.]] | [[Category: Hendrickson, W A.]] | ||
[[Category: Peat, T S.]] | [[Category: Peat, T S.]] | ||
| - | [[Category: | + | [[Category: Beta-lactamase cleavage reaction]] |
| - | [[Category: | + | [[Category: Dna repair]] |
| - | [[Category: | + | [[Category: Induced mutagenesis]] |
| - | [[Category: | + | [[Category: Lambda ci]] |
| - | [[Category: | + | [[Category: Lexa repressor]] |
| - | [[Category: | + | [[Category: Sos mutagenesis]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:26:34 2008'' | |
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Revision as of 08:26, 3 May 2008
STRUCTURE DETERMINATION OF UMUD' BY MAD PHASING OF THE SELENOMETHIONYL PROTEIN
Overview
For life to be sustained, mistakes in DNA repair must be tolerated when damage obscures the genetic information. In bacteria such as Escherichia coli, DNA damage elicits the well regulated 'SOS response'. For the extreme case of damage that cannot be repaired by conventional enzymes, there are proteins that allow the replication of DNA through such lesions, but with a reduction in the fidelity of replication. Essential proteins in this mutagenic process are RecA, DNA polymerase III, UmuD, UmuD' and UmuC (umu: UV mutagenesis). Regulation of this response involves a RecA-mediated self-cleavage of UmuD to produce UmuD'. To understand this system in more detail, we have determined the crystal structure of the E. coli UmuD' mutagenesis protein at 2.5 A resolution. Globular heads folded in an unusual Beta-structure associate to form molecular dimers, and extended amino-terminal tails associate to produce crystallized filaments. The structure provides insight into the mechanism of the self-cleavage reaction that UmuD-like proteins undergo as part of the global SOS response.
About this Structure
1UMU is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of the UmuD' protein and its regulation in response to DNA damage., Peat TS, Frank EG, McDonald JP, Levine AS, Woodgate R, Hendrickson WA, Nature. 1996 Apr 25;380(6576):727-30. PMID:8614470 Page seeded by OCA on Sat May 3 11:26:34 2008
