1umz

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[[Image:1umz.gif|left|200px]]
[[Image:1umz.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1umz |SIZE=350|CAPTION= <scene name='initialview01'>1umz</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1umz", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Gal+Binding+Site+For+Chain+B'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Xyloglucan:xyloglucosyl_transferase Xyloglucan:xyloglucosyl transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.207 2.4.1.207] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1umz| PDB=1umz | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1umz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1umz OCA], [http://www.ebi.ac.uk/pdbsum/1umz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1umz RCSB]</span>
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}}
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'''XYLOGLUCAN ENDOTRANSGLYCOSYLASE IN COMPLEX WITH THE XYLOGLUCAN NONASACCHARIDE XLLG.'''
'''XYLOGLUCAN ENDOTRANSGLYCOSYLASE IN COMPLEX WITH THE XYLOGLUCAN NONASACCHARIDE XLLG.'''
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[[Category: Kallas, A M.]]
[[Category: Kallas, A M.]]
[[Category: Teeri, T T.]]
[[Category: Teeri, T T.]]
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[[Category: glycoside hydrolase]]
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[[Category: Glycoside hydrolase]]
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[[Category: glycosyltransferase]]
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[[Category: Glycosyltransferase]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: transglycosylation]]
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[[Category: Transglycosylation]]
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[[Category: xeh]]
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[[Category: Xeh]]
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[[Category: xet]]
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[[Category: Xet]]
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[[Category: xth]]
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[[Category: Xth]]
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[[Category: xyloglucan]]
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[[Category: Xyloglucan]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:26:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:12:31 2008''
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Revision as of 08:26, 3 May 2008

Image:1umz.gif

Template:STRUCTURE 1umz

XYLOGLUCAN ENDOTRANSGLYCOSYLASE IN COMPLEX WITH THE XYLOGLUCAN NONASACCHARIDE XLLG.


Overview

Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Thus, XET is a key enzyme in all plant processes that require cell wall remodeling. To provide a basis for detailed structure-function studies, the crystal structure of Populus tremula x tremuloides XET16A (PttXET16A), heterologously expressed in Pichia pastoris, has been determined at 1.8-A resolution. Even though the overall structure of PttXET16A is a curved beta-sandwich similar to other enzymes in the glycoside hydrolase family GH16, parts of its substrate binding cleft are more reminiscent of the distantly related family GH7. In addition, XET has a C-terminal extension that packs against the conserved core, providing an additional beta-strand and a short alpha-helix. The structure of XET in complex with a xyloglucan nonasaccharide, XLLG, reveals a very favorable acceptor binding site, which is a necessary but not sufficient prerequisite for transglycosylation. Biochemical data imply that the enzyme requires sugar residues in both acceptor and donor sites to properly orient the glycosidic bond relative to the catalytic residues.

About this Structure

1UMZ is a Single protein structure of sequence from Populus tremula. Full crystallographic information is available from OCA.

Reference

Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding., Johansson P, Brumer H 3rd, Baumann MJ, Kallas AM, Henriksson H, Denman SE, Teeri TT, Jones TA, Plant Cell. 2004 Apr;16(4):874-86. Epub 2004 Mar 12. PMID:15020748 Page seeded by OCA on Sat May 3 11:26:47 2008

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