9j61

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Current revision (06:23, 4 December 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 9j61 is ON HOLD until Paper Publication
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==Crystal structure of a cyclodipeptide synthase from Streptomyces sapporonensis==
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<StructureSection load='9j61' size='340' side='right'caption='[[9j61]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[9j61]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cinnamoneus Streptomyces cinnamoneus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9J61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9J61 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9j61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9j61 OCA], [https://pdbe.org/9j61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9j61 RCSB], [https://www.ebi.ac.uk/pdbsum/9j61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9j61 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A2G1XAZ9_STRCJ A0A2G1XAZ9_STRCJ]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cyclodipeptide synthases (CDPSs) catalyze the synthesis of diverse cyclodipeptides (CDPs) by utilizing two aminoacyl-tRNA (aa-tRNA) substrates in a sequential ping-pong reaction mechanism. Numerous CDPSs have been characterized to provide precursors for diketopiperazines (DKPs) with diverse structural characteristics and biological activities. BcmA, belonging to the XYP subfamily, is a cyclo(l-Ile-l-Leu)-synthesizing CDPS involved in the biosynthesis of the antibiotic bicyclomycin. The structural basis and determinants influencing BcmA enzyme activity and substrate selectivity are not well understood. Here, we report the crystal structure of SsBcmA from Streptomyces sapporonensis. Through structural comparison and systematic site-directed mutagenesis, we highlight the significance of key residues located in the aminoacyl-binding pocket for enzyme activity and substrate specificity. In particular, the nonconserved residues D161 and K165 in pocket P2 are essential for the activity of SsBcmA without significant alteration of the substrate specificity, while the conserved residues F158 as well as F210 and S211 in P2 are responsible for determining substrate selectivity. These findings facilitate the understanding of how CDPSs selectively accept hydrophobic substrates and provide additional clues for the engineering of these enzymes for synthetic biology applications.
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Authors:
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Crystal Structure and Mutagenesis of an XYP Subfamily Cyclodipeptide Synthase Reveal Key Determinants of Enzyme Activity and Substrate Specificity.,He JB, Ren Y, Li P, Liu YP, Pan HX, Huang LJ, Wang J, Fang P, Tang GL Biochemistry. 2024 Nov 19;63(22):2969-2976. doi: 10.1021/acs.biochem.4c00505. , Epub 2024 Oct 30. PMID:39475147<ref>PMID:39475147</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 9j61" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptomyces cinnamoneus]]
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[[Category: Fang P]]
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[[Category: He J]]
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[[Category: Li P]]
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[[Category: Ren Y]]
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[[Category: Tang G]]
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[[Category: Wang J]]
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[[Category: Wu S]]

Current revision

Crystal structure of a cyclodipeptide synthase from Streptomyces sapporonensis

PDB ID 9j61

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