8r2t
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of 4-hydroxybenzoate-1-hydroxylase from Gelatoporia subvermispora (GsMNX1)== | |
| + | <StructureSection load='8r2t' size='340' side='right'caption='[[8r2t]], [[Resolution|resolution]] 1.82Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8r2t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gelatoporia_subvermispora Gelatoporia subvermispora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8R2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8R2T FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.824Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8r2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8r2t OCA], [https://pdbe.org/8r2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8r2t RCSB], [https://www.ebi.ac.uk/pdbsum/8r2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8r2t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/M2QGN5_CERS8 M2QGN5_CERS8] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | White-rot fungi (WRF) are the most efficient lignin-degrading organisms in nature. However, their capacity to use lignin-related aromatic compounds, such as 4-hydroxybenzoate, as carbon sources has only been described recently. Previously, the hydroxyquinol pathway was proposed for the bioconversion of these compounds in fungi, but gene- and structure-function relationships of the full enzymatic pathway remain uncharacterized in any single fungal species. Here, we characterize seven enzymes from two WRF, Trametes versicolor and Gelatoporia subvermispora, which constitute a four-enzyme cascade from 4-hydroxybenzoate to beta-ketoadipate via the hydroxyquinol pathway. Furthermore, we solve the crystal structure of four of these enzymes and identify mechanistic differences with the closest bacterial and fungal structural homologs. Overall, this research expands our understanding of aromatic catabolism by WRF and establishes an alternative strategy for the conversion of lignin-related compounds to the valuable molecule beta-ketoadipate, contributing to the development of biological processes for lignin valorization. | ||
| - | + | Biochemical and structural characterization of enzymes in the 4-hydroxybenzoate catabolic pathway of lignin-degrading white-rot fungi.,Kuatsjah E, Schwartz A, Zahn M, Tornesakis K, Kellermyer ZA, Ingraham MA, Woodworth SP, Ramirez KJ, Cox PA, Pickford AR, Salvachua D Cell Rep. 2024 Nov 25;43(12):115002. doi: 10.1016/j.celrep.2024.115002. PMID:39589922<ref>PMID:39589922</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8r2t" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gelatoporia subvermispora]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kuatsjah E]] | ||
| + | [[Category: Salvachua D]] | ||
| + | [[Category: Zahn M]] | ||
Current revision
Crystal structure of 4-hydroxybenzoate-1-hydroxylase from Gelatoporia subvermispora (GsMNX1)
| |||||||||||
