8xwb
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of dinitrosyl iron units binding with human heavy chain Ferritin== | |
| + | <StructureSection load='8xwb' size='340' side='right'caption='[[8xwb]], [[Resolution|resolution]] 1.69Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8xwb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XWB FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xwb OCA], [https://pdbe.org/8xwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xwb RCSB], [https://www.ebi.ac.uk/pdbsum/8xwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xwb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/FRIH_HUMAN FRIH_HUMAN] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Iron-sulfur cluster conversion and nitrosyl modification are involved in regulating their functions and play critical roles in signaling for biological systems. Hereby, the photo-induced dynamic process of (Me(4)N)(2)[Fe(2)S(2)(NO)(4)] was monitored using time-resolved electron paramagnetic resonance (EPR) spectra, MS spectra and cellular imaging methods. Photo-irradiation and the solvent affect the reaction rates and products. Spectroscopic and kinetic studies have shown that the process involves at least three intermediates: spin-trapped NO free radical species with a g(av) at 2.040, and two other iron nitrosyl species, dinitrosyl iron units (DNICs) and mononitrosyl iron units (MNICs) with g(av) values at 2.031 and 2.024, respectively. Moreover, the [Fe(2)S(2)(NO)(4)](2-) cluster could bind with ferritin and decompose gradually, and a binding state of dinitrosyl iron coordinated with Cys102 of the recombinant human heavy chain ferritin (rHuHF) was finally formed. This study provides insight into the photodynamic mechanism of nitrosyl iron - sulfur clusters to improve the understanding of physiological activity. | ||
| - | + | Insight into the photodynamic mechanism and protein binding of a nitrosyl iron-sulfur [Fe(2)S(2)(NO)(4)](2-) cluster.,Gong W, Wu T, Liu Y, Jiao S, Wang W, Yan W, Li Y, Liu Y, Zhang Y, Wang H Spectrochim Acta A Mol Biomol Spectrosc. 2024 Nov 5;320:124603. doi: , 10.1016/j.saa.2024.124603. Epub 2024 Jun 5. PMID:38878720<ref>PMID:38878720</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8xwb" style="background-color:#fffaf0;"></div> |
| - | [[Category: Wang | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Gong WJ]] | ||
| + | [[Category: Wang HF]] | ||
| + | [[Category: Wang WM]] | ||
Current revision
Crystal structure of dinitrosyl iron units binding with human heavy chain Ferritin
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