1uph
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1uph.gif|left|200px]] | [[Image:1uph.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1uph", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1uph| PDB=1uph | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''HIV-1 MYRISTOYLATED MATRIX''' | '''HIV-1 MYRISTOYLATED MATRIX''' | ||
| Line 23: | Line 20: | ||
==Reference== | ==Reference== | ||
Entropic switch regulates myristate exposure in the HIV-1 matrix protein., Tang C, Loeliger E, Luncsford P, Kinde I, Beckett D, Summers MF, Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):517-22. Epub 2003 Dec 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14699046 14699046] | Entropic switch regulates myristate exposure in the HIV-1 matrix protein., Tang C, Loeliger E, Luncsford P, Kinde I, Beckett D, Summers MF, Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):517-22. Epub 2003 Dec 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14699046 14699046] | ||
| - | [[Category: Human immunodeficiency virus type 1 (isolate 12)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Beckett, D.]] | [[Category: Beckett, D.]] | ||
| Line 31: | Line 27: | ||
[[Category: Summers, M F.]] | [[Category: Summers, M F.]] | ||
[[Category: Tang, C.]] | [[Category: Tang, C.]] | ||
| - | [[Category: | + | [[Category: Myristoylated]] |
| - | [[Category: | + | [[Category: Myristyl]] |
| - | [[Category: | + | [[Category: Myristyl switch]] |
| - | [[Category: | + | [[Category: Phosphorylation]] |
| - | [[Category: | + | [[Category: Polyprotein]] |
| - | [[Category: | + | [[Category: Post-translational modification]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:32:06 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:32, 3 May 2008
HIV-1 MYRISTOYLATED MATRIX
Overview
The myristoylated matrix protein (myr-MA) of HIV functions as a regulator of intracellular localization, targeting the Gag precursor polyprotein to lipid rafts in the plasma membrane during virus assembly and dissociating from the membrane during infectivity for nuclear targeting of the preintegration complex. Membrane release is triggered by proteolytic cleavage of Gag, and it has, until now, been believed that proteolysis induces a conformational change in myr-MA that sequesters the myristyl group. NMR studies reported here reveal that myr-MA adopts myr-exposed [myr(e)] and -sequestered [myr(s)] states, as anticipated. Unexpectedly, the tertiary structures of the protein in both states are very similar, with the sequestered myristyl group occupying a cavity that requires only minor conformational adjustments for insertion. In addition, myristate exposure is coupled with trimerization, with the myristyl group sequestered in the monomer and exposed in the trimer (K(assoc) = 2.5 +/- 0.6 x 10(8) M(-2)). The equilibrium constant is shifted approximately 20-fold toward the trimeric, myristate-exposed species in a Gag-like construct that includes the capsid domain, indicating that exposure is enhanced by Gag subdomains that promote self-association. Our findings indicate that the HIV-1 myristyl switch is regulated not by mechanically induced conformational changes, as observed for other myristyl switches, but instead by entropic modulation of a preexisting equilibrium.
About this Structure
1UPH is a Single protein structure of sequence from Human immunodeficiency virus type 1 (isolate 12). Full crystallographic information is available from OCA.
Reference
Entropic switch regulates myristate exposure in the HIV-1 matrix protein., Tang C, Loeliger E, Luncsford P, Kinde I, Beckett D, Summers MF, Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):517-22. Epub 2003 Dec 29. PMID:14699046 Page seeded by OCA on Sat May 3 11:32:06 2008
