9cuf
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Room temperature SSX structure of ccNiR== | |
| + | <StructureSection load='9cuf' size='340' side='right'caption='[[9cuf]], [[Resolution|resolution]] 3.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9cuf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis Shewanella oneidensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9CUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9CUF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=PE3:3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL'>PE3</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9cuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9cuf OCA], [https://pdbe.org/9cuf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9cuf RCSB], [https://www.ebi.ac.uk/pdbsum/9cuf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9cuf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NRFA_SHEON NRFA_SHEON] Plays a role in nitrite reduction.[HAMAP-Rule:MF_01182] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The upgrade of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France to an Extremely Brilliant Source (EBS) is expected to enable time-resolved synchrotron serial crystallography (SSX) experiments with sub-millisecond time resolution. ID29 is a new beamline dedicated to SSX experiments at ESRF-EBS. Here, we report experiments emerging from the initial phase of user operation at ID29. We first used microcrystals of photoactive yellow protein as a model system to exploit the potential of microsecond pulses for SSX. Subsequently, we investigated microcrystals of cytochrome c nitrite reductase (ccNiR) with microsecond X-ray pulses. CcNiR is a decaheme protein that is ideal for the investigation of radiation damage at the various heme-iron sites. Finally, we performed a proof-of-concept subsecond time-resolved SSX experiment by photoactivating microcrystals of a myxobacterial phytochrome. | ||
| - | + | Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.,Malla TN, Muniyappan S, Menendez D, Ogukwe F, Dale AN, Clayton JD, Weatherall DD, Karki P, Dangi S, Mandella V, Pacheco AA, Stojkovic EA, Rose SL, Orlans J, Basu S, de Sanctis D, Schmidt M IUCrJ. 2025 Jan 1;12(Pt 1):36-48. doi: 10.1107/S2052252524010868. PMID:39575537<ref>PMID:39575537</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9cuf" style="background-color:#fffaf0;"></div> |
| - | [[Category: Malla | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Shewanella oneidensis]] | ||
| + | [[Category: Malla TN]] | ||
| + | [[Category: Schmidt M]] | ||
Current revision
Room temperature SSX structure of ccNiR
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