9h9q
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Candida albicans gamma-tubulin small complex within ring-like higher oligomer in complex with Spc72 CM1== | |
| + | <StructureSection load='9h9q' size='340' side='right'caption='[[9h9q]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9h9q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9H9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9H9Q FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9h9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9h9q OCA], [https://pdbe.org/9h9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9h9q RCSB], [https://www.ebi.ac.uk/pdbsum/9h9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9h9q ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A8H6F519_CANAX A0A8H6F519_CANAX] Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome.[RuleBase:RU000352] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The gamma-tubulin ring complex (gamma-TuRC) is a structural template for controlled nucleation of microtubules from alpha/beta-tubulin heterodimers. At the cytoplasmic side of the yeast spindle pole body, the CM1-containing receptor protein Spc72 promotes gamma-TuRC assembly from seven gamma-tubulin small complexes (gamma-TuSCs) and recruits the microtubule polymerase Stu2, yet their molecular interplay remains unclear. Here, we determine the cryo-EM structure of the Candida albicans cytoplasmic nucleation unit at 3.6 A resolution, revealing how the gamma-TuRC is assembled and conformationally primed for microtubule nucleation by the dimerised Spc72 CM1 motif. Two coiled-coil regions of Spc72 interact with the conserved C-terminal alpha-helix of Stu2 and thereby position the alpha/beta-tubulin-binding TOG domains of Stu2 in the vicinity of the microtubule assembly site. Collectively, we reveal the function of CM1 motifs in gamma-TuSC oligomerisation and the recruitment of microtubule polymerases to the gamma-TuRC. | ||
| - | + | Structural insights into the interplay between microtubule polymerases, gamma-tubulin complexes and their receptors.,Zheng A, Vermeulen BJA, Wurtz M, Neuner A, Lubbehusen N, Mayer MP, Schiebel E, Pfeffer S Nat Commun. 2025 Jan 5;16(1):402. doi: 10.1038/s41467-024-55778-7. PMID:39757296<ref>PMID:39757296</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9h9q" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Candida albicans]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pfeffer S]] | ||
| + | [[Category: Vermeulen BJA]] | ||
Current revision
Candida albicans gamma-tubulin small complex within ring-like higher oligomer in complex with Spc72 CM1
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