9iqb
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of beta-glucosidase from Acetivibrio thermocellus== | |
| + | <StructureSection load='9iqb' size='340' side='right'caption='[[9iqb]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9iqb]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IQB FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9iqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9iqb OCA], [https://pdbe.org/9iqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9iqb RCSB], [https://www.ebi.ac.uk/pdbsum/9iqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9iqb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/BGLA_ACET2 BGLA_ACET2] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cellulases are an ensemble of enzymes that hydrolyze cellulose chains into fermentable glucose and hence are widely used in bioethanol production. The last enzyme of the cellulose degradation pathway, beta-glucosidase, is inhibited by its product, glucose. The product inhibition by glucose hinders cellulose hydrolysis limiting the saccharification during bioethanol production. Thus, engineered beta-glucosidases with enhanced glucose tolerance and catalytic efficiency are essential. This study focuses on the rational engineering of beta-glucosidase from Acetivibrio thermocellus (WT-AtGH1). Recombinant WT-AtGH1 exhibited activity on cellobiose and p-nitrophenyl-beta-d-glucoside as substrates and retained around 80% of its activity over 48 h at 55 degrees C, pH 5.5. However, WT-AtGH1 showed low glucose tolerance of 380 mm as compared to the required IC(50) value of > 800 mm for industrial use. Thus, a rational design approach was utilized to enhance the glucose tolerance of this enzyme. We determined the 3 A resolution crystal structure of WT-AtGH1. The structure-based engineered G168W-AtGH1 and S242W-AtGH1 mutants exhibited improved glucose tolerance of 840 and 612 mm, respectively. Surprisingly, S242L-AtGH1 mutant showed ~ 2.5-fold increase in the catalytic efficiency as compared to WT-AtGH1. A combinatorial effect of improved glucose tolerance, as well as enhanced catalytic efficiency, was observed for the G168W-S242L-AtGH1 mutant. All the mutants with enhanced properties showed considerable stability at industrial operating conditions of 55 degrees C and pH 5.5. Thus, we present mutants of WT-AtGH1 with improved glucose tolerance and kinetic properties that have the potential to increase the efficiency of saccharification during biofuel production. | ||
| - | + | Rational design facilitates the improvement of glucose tolerance and catalytic properties of a beta-glucosidase from Acetivibrio thermocellus.,Kamale C, Rauniyar A, Bhaumik P FEBS J. 2025 Jan 7. doi: 10.1111/febs.17394. PMID:39764622<ref>PMID:39764622</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9iqb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Acetivibrio thermocellus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bhaumik P]] | ||
| + | [[Category: Kamale C]] | ||
Current revision
Crystal structure of beta-glucosidase from Acetivibrio thermocellus
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