1ux7
From Proteopedia
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[[Image:1ux7.gif|left|200px]] | [[Image:1ux7.gif|left|200px]] | ||
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'''CARBOHYDRATE-BINDING MODULE CBM36 IN COMPLEX WITH CALCIUM AND XYLOTRIOSE''' | '''CARBOHYDRATE-BINDING MODULE CBM36 IN COMPLEX WITH CALCIUM AND XYLOTRIOSE''' | ||
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[[Category: Davies, G J.]] | [[Category: Davies, G J.]] | ||
[[Category: Jamal, S.]] | [[Category: Jamal, S.]] | ||
| - | [[Category: | + | [[Category: Calcium]] |
| - | [[Category: | + | [[Category: Carbohydrate binding domain]] |
| - | [[Category: | + | [[Category: Carbohydrate-binding module cbm36]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Xylan]] |
| - | [[Category: | + | [[Category: Xylanase gh43]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:48:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:48, 3 May 2008
CARBOHYDRATE-BINDING MODULE CBM36 IN COMPLEX WITH CALCIUM AND XYLOTRIOSE
Overview
The enzymatic degradation of polysaccharides harnesses multimodular enzymes whose carbohydrate binding modules (CBM) target the catalytic domain onto the recalcitrant substrate. Here we report the ab initio structure determination and subsequent refinement, at 0.8 A resolution, of the CBM36 domain of the Paenibacillus polymyxa xylanase 43A. Affinity electrophoresis, isothermal titration calorimetry, and UV difference spectroscopy demonstrate that CBM36 is a novel Ca(2+)-dependent xylan binding domain. The 3D structure of CBM36 in complex with xylotriose and Ca(2+), at 1.5 A resolution, displays significant conformational changes compared to the native structure and reveals the molecular basis for its unique Ca(2+)-dependent binding of xylooligosaccharides through coordination of the O2 and O3 hydroxyls. CBM36 is one of an emerging spectrum of carbohydrate binding modules that increasingly find applications in industry and display great potential for mapping the "glyco-architecture" of plant cells.
About this Structure
1UX7 is a Single protein structure of sequence from Paenibacillus polymyxa. Full crystallographic information is available from OCA.
Reference
Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules., Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ, Structure. 2004 Jul;12(7):1177-87. PMID:15242594 Page seeded by OCA on Sat May 3 11:48:22 2008
