9h3r
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==50S subunit precursor C-CP_YjgA_(L22)-== | |
| + | <StructureSection load='9h3r' size='340' side='right'caption='[[9h3r]], [[Resolution|resolution]] 4.12Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9h3r]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9H3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9H3R FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.12Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9h3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9h3r OCA], [https://pdbe.org/9h3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9h3r RCSB], [https://www.ebi.ac.uk/pdbsum/9h3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9h3r ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DARP_ECOLI DARP_ECOLI] Member of a network of 50S ribosomal subunit biogenesis factors (ObgE, RluD, RsfS and DarP) which assembles along the 30S-50S interface, preventing incorrect 23S rRNA structures from forming (PubMed:33639093). Promotes peptidyl transferase center (PTC) maturation (PubMed:33639093). Binds pre-50S ribosomal subunits near the 23S rRNA L1 stalk, where it may mediate correct positioning of the L1 stalk (PubMed:33639093). Plays a dual role in the late stages of assembly of the 50S ribosomal subunit; promotes peptidyl transferase center (PTC) maturation by modulating the docking of 23S rRNA helix H68, and maintains the stability of helix H89 with the help of uL16 (rplP), facilitating the final maturation of the PTC (Probable) (PubMed:38842932). Overexpression slows growth at 37 degrees Celsius (PubMed:38842932).[HAMAP-Rule:MF_00765]<ref>PMID:33639093</ref> <ref>PMID:38842932</ref> <ref>PMID:38842932</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The proline-rich antimicrobial designer peptide Api137 inhibits protein expression in bacteria by binding simultaneously to the ribosomal polypeptide exit tunnel and the release factor (RF), depleting the cellular RF pool and leading to ribosomal arrest at stop codons. This study investigates the additional effect of Api137 on the assembly of ribosomes using an Escherichia coli reporter strain expressing one ribosomal protein per 30S and 50S subunit tagged with mCherry and EGFP, respectively. Separation of cellular extracts derived from cells exposed to Api137 in a sucrose gradient reveals elevated levels of partially assembled and not fully matured precursors of the 50S subunit (pre-50S). High-resolution structures obtained by cryogenic electron microscopy demonstrate that a large proportion of pre-50S states are missing up to five proteins (uL22, bL32, uL29, bL23, and uL16) and have misfolded helices in 23S rRNA domain IV. These data suggest a second mechanism for Api137, wherein it disrupts 50S subunit assembly by inducing the formation of misfolded precursor particles potentially incapable of evolving into active ribosomes, suggesting a bactericidal mechanism. | ||
| - | + | The proline-rich antimicrobial peptide Api137 disrupts large ribosomal subunit assembly and induces misfolding.,Lauer SM, Gasse J, Krizsan A, Reepmeyer M, Sprink T, Nikolay R, Spahn CMT, Hoffmann R Nat Commun. 2025 Jan 10;16(1):567. doi: 10.1038/s41467-025-55836-8. PMID:39794318<ref>PMID:39794318</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9h3r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lauer S]] | ||
| + | [[Category: Nikolay R]] | ||
| + | [[Category: Spahn CMT]] | ||
Current revision
50S subunit precursor C-CP_YjgA_(L22)-
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