1uzw

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[[Image:1uzw.gif|left|200px]]
[[Image:1uzw.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1uzw |SIZE=350|CAPTION= <scene name='initialview01'>1uzw</scene>, resolution 1.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1uzw", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CDH:D-(L-A-AMINOADIPOYL)-L-CYSTEINYL-D-ISODEHYDROVALINE'>CDH</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Isopenicillin-N_synthase Isopenicillin-N synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1uzw| PDB=1uzw | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uzw OCA], [http://www.ebi.ac.uk/pdbsum/1uzw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uzw RCSB]</span>
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}}
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'''ISOPENICILLIN N SYNTHASE WITH L-D-(A-AMINOADIPOYL)-L-CYSTEINYL-D-ISODEHYDROVALINE'''
'''ISOPENICILLIN N SYNTHASE WITH L-D-(A-AMINOADIPOYL)-L-CYSTEINYL-D-ISODEHYDROVALINE'''
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[[Category: Grummitt, A R.]]
[[Category: Grummitt, A R.]]
[[Category: Rutledge, P J.]]
[[Category: Rutledge, P J.]]
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[[Category: antibiotic biosynthesis]]
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[[Category: Antibiotic biosynthesis]]
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[[Category: b-lactam antibiotic]]
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[[Category: B-lactam antibiotic]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: oxygenase]]
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[[Category: Oxygenase]]
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[[Category: penicillin biosynthesis]]
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[[Category: Penicillin biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:55:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:17:42 2008''
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Revision as of 08:55, 3 May 2008

Image:1uzw.gif

Template:STRUCTURE 1uzw

ISOPENICILLIN N SYNTHASE WITH L-D-(A-AMINOADIPOYL)-L-CYSTEINYL-D-ISODEHYDROVALINE


Overview

Isopenicillin N synthase (IPNS) is a non-haem iron oxidase that catalyses the formation of bicyclic isopenicillin N from delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV). In this study we report a novel activity for the iron of the IPNS active site, which behaves as a Lewis acid to catalyse the elimination of HF from the fluorinated substrate analogue, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-beta-fluorovaline (ACbetaFV). X-Ray crystallographic studies of IPNS crystals grown anaerobically with ACbetaFV reveal that the valinyl beta-fluorine is missing from the active site region, and suggest the presence of the unsaturated tripeptide delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-isodehydrovaline in place of substrate ACbetaFV. (19)F NMR studies confirm the release of fluoride from ACbetaFV in the presence of the active IPNS enzyme. These results suggest a new mode of reactivity for the IPNS iron centre, a mechanism of action that has not previously been reported for any of the iron oxidase enzymes.

About this Structure

1UZW is a Single protein structure of sequence from Emericella nidulans. Full crystallographic information is available from OCA.

Reference

Active-site-mediated elimination of hydrogen fluoride from a fluorinated substrate analogue by isopenicillin N synthase., Grummitt AR, Rutledge PJ, Clifton IJ, Baldwin JE, Biochem J. 2004 Sep 1;382(Pt 2):659-66. PMID:15175003 Page seeded by OCA on Sat May 3 11:55:03 2008

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