8y9q
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==b-glucosidase from Thermotoga profunda Tp-BGL== | |
| + | <StructureSection load='8y9q' size='340' side='right'caption='[[8y9q]], [[Resolution|resolution]] 1.96Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8y9q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_profunda Thermotoga profunda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Y9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Y9Q FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8y9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8y9q OCA], [https://pdbe.org/8y9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8y9q RCSB], [https://www.ebi.ac.uk/pdbsum/8y9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8y9q ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | beta-Glucosidase is a crucial cellulase, as its activity determines the efficiency of cellulose hydrolysis into glucose. This study addresses the functional and structural characteristics of Thermotoga profunda beta-glucosidase (Tp-BGL). Tp-BGL exhibited a K(m) of 0.3798 mM for p-nitrophenyl-beta-d-glucopyranoside (pNPGlc) and 4.44 mM for cellobiose, with k(cat)/K(m) of 1211.16 and 4.18 s(-1) mM(-1), respectively. In addition, Tp-BGL showed significant pH adaptability and thermal stability, with a T(m) of 85.7 degrees C and retaining >90 % of its activity after incubation at 80 degrees C for 90 min. The crystal structure of Tp-BGL was resolved at 1.95 A resolution, and reveals a typical TIM barrel structure. Comparative structural analysis highlighted that the major distinction between Tp-BGL and the other glucosidases lies in their loop regions. | ||
| - | + | Structural insights and functional characterization of a novel beta-glucosidase derived from Thermotoga profunda.,Guo Y, Chen A, Liu K, Ji C Biochem Biophys Res Commun. 2024 Nov 5;732:150405. doi: , 10.1016/j.bbrc.2024.150405. Epub 2024 Jul 14. PMID:39033552<ref>PMID:39033552</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8y9q" style="background-color:#fffaf0;"></div> |
| - | [[Category: Chen | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermotoga profunda]] | ||
| + | [[Category: Chen A]] | ||
| + | [[Category: Guo Y]] | ||
Current revision
b-glucosidase from Thermotoga profunda Tp-BGL
| |||||||||||
