9g7i
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) in complex with acetyl-Coenyzme A from Clostridium autoethanogenum== | |
| + | <StructureSection load='9g7i' size='340' side='right'caption='[[9g7i]], [[Resolution|resolution]] 2.93Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9g7i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_autoethanogenum_DSM_10061 Clostridium autoethanogenum DSM 10061]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9G7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9G7I FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.93Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9g7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9g7i OCA], [https://pdbe.org/9g7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9g7i RCSB], [https://www.ebi.ac.uk/pdbsum/9g7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9g7i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/F8TEQ9_9CLOT F8TEQ9_9CLOT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In the ancient microbial Wood-Ljungdahl pathway, carbon dioxide (CO(2)) is fixed in a multistep process that ends with acetyl-coenzyme A (acetyl-CoA) synthesis at the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase complex (CODH/ACS). In this work, we present structural snapshots of the CODH/ACS from the gas-converting acetogen Clostridium autoethanogenum, characterizing the molecular choreography of the overall reaction, including electron transfer to the CODH for CO(2) reduction, methyl transfer from the corrinoid iron-sulfur protein (CoFeSP) partner to the ACS active site, and acetyl-CoA production. Unlike CODH, the multidomain ACS undergoes large conformational changes to form an internal connection to the CODH active site, accommodate the CoFeSP for methyl transfer, and protect the reaction intermediates. Altogether, the structures allow us to draw a detailed reaction mechanism of this enzyme, which is crucial for CO(2) fixation in anaerobic organisms. | ||
| - | + | Conformational dynamics of a multienzyme complex in anaerobic carbon fixation.,Yin MD, Lemaire ON, Rosas Jimenez JG, Belhamri M, Shevchenko A, Hummer G, Wagner T, Murphy BJ Science. 2025 Jan 31;387(6733):498-504. doi: 10.1126/science.adr9672. Epub 2025 , Jan 30. PMID:39883773<ref>PMID:39883773</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9g7i" style="background-color:#fffaf0;"></div> |
| - | [[Category: Murphy | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Yin | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Clostridium autoethanogenum DSM 10061]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lemaire ON]] | ||
| + | [[Category: Murphy BJ]] | ||
| + | [[Category: Wagner T]] | ||
| + | [[Category: Yin MD]] | ||
Current revision
Structure of carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) in complex with acetyl-Coenyzme A from Clostridium autoethanogenum
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