8ia1
From Proteopedia
(Difference between revisions)
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==plastidial glycerol-3-phosphate acyltransferases (GPAT) from the green alga Myrmecia incisa== | ==plastidial glycerol-3-phosphate acyltransferases (GPAT) from the green alga Myrmecia incisa== | ||
| - | <StructureSection load='8ia1' size='340' side='right'caption='[[8ia1]]' scene=''> | + | <StructureSection load='8ia1' size='340' side='right'caption='[[8ia1]], [[Resolution|resolution]] 2.31Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IA1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[8ia1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobosphaera_incisa Lobosphaera incisa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IA1 FirstGlance]. <br> |
| - | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ia1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ia1 OCA], [https://pdbe.org/8ia1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ia1 RCSB], [https://www.ebi.ac.uk/pdbsum/8ia1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ia1 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ia1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ia1 OCA], [https://pdbe.org/8ia1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ia1 RCSB], [https://www.ebi.ac.uk/pdbsum/8ia1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ia1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Plastidial glycerol-3-phosphate acyltransferases (GPATs) catalyze acyl-ACP and glycerol-3-phosphate to synthesize lysophosphatidic acid in vivo, which initiates the formation of various glycerolipids. Although the physiological substrates of plastidial GPATs are acyl-ACPs, acyl-CoAs have been commonly studied on the GPATs in vitro. However, little is known whether there are any distinct features of GPATs towards acyl-ACP and acyl-CoA. In this study, the results showed that the microalgal plastidial GPATs preferred acyl-ACP to acyl-CoA, while surprisingly, the plant-derived plastidial GPATs showed no obvious preferences towards these two acyl carriers. The key residues responsible for the distinct feature of microalgal plastidial GPATs were compared with plant-derived plastidial GPATs in their efficiency to catalyze acyl-ACP and acyl-CoA. Microalgal plastidial GPATs uniquely recognized acyl-ACP as compared to with other acyltransferases. The structure of the acyltransferases-ACP complex highlights only the involvement of the large structural domain in ACP in microalgal plastidial GPAT while in the other acyltransferases, both large and small structural domains were involved in the recognition process. The interaction sites on the plastidial GPAT from the green alga Myrmecia incisa (MiGPAT1) with ACP turned out to be K204, R212 and R266. A unique recognition between the microalgal plastidial GPAT and ACP was elucidated. | ||
| + | |||
| + | Unique recognition of the microalgal plastidial glycerol-3-phosphate acyltransferase for acyl-ACP.,Li X, Yang M, Sun D, Shi J, Yang M, Feng Y, Xue S Plant Sci. 2023 Jul;332:111725. doi: 10.1016/j.plantsci.2023.111725. Epub 2023 , May 3. PMID:37142097<ref>PMID:37142097</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8ia1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Song | + | [[Category: Lobosphaera incisa]] |
| + | [[Category: Song X]] | ||
Current revision
plastidial glycerol-3-phosphate acyltransferases (GPAT) from the green alga Myrmecia incisa
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