9cr6
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of histidine racemase (HisR) of Fusobacterium nucleatum (C209S)== | |
| + | <StructureSection load='9cr6' size='340' side='right'caption='[[9cr6]], [[Resolution|resolution]] 2.49Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9cr6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusobacterium_nucleatum Fusobacterium nucleatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9CR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9CR6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9cr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9cr6 OCA], [https://pdbe.org/9cr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9cr6 RCSB], [https://www.ebi.ac.uk/pdbsum/9cr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9cr6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8RI81_FUSNN Q8RI81_FUSNN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Fusobacterium nucleatum is an oral commensal bacterium that can act as an opportunistic pathogen and is implicated in diseases such as periodontitis, adverse pregnancy outcomes, colorectal cancer, and Alzheimer's disease. F. nucleatum synthesizes lanthionine for its peptidoglycan, rather than meso-2,6-diaminopimelic acid (DAP) used by most Gram-negative bacteria. Despite lacking the biosynthetic pathway for DAP, the genome of F. nucleatum ATCC 25586 encodes a predicted DAP epimerase. A recent study hypothesized that this enzyme may act as a lanthionine epimerase, but the authors found a very low turnover rate, suggesting that this enzyme likely has another more favored substrate. Here, we characterize this enzyme as a histidine racemase (HisR), and found that catalytic turnover is approximately 10,000x faster with L-histidine than with L,L-lanthionine. Kinetic experiments suggest that HisR functions as a cofactor-independent racemase and that turnover is specific for histidine, while crystal structures of catalytic cysteine to serine mutants (C67S or C209S) reveal this enzyme in its substrate-unbound, open conformation. Currently, the only other reported cofactor-independent histidine racemase is CntK from Staphylococcus aureus, which is used in the biosynthesis of staphylopine, a broad-spectrum metallophore that increases virulence of S. aureus. However, CntK shares only 28% sequence identity with HisR, and their genes exist in different genomic contexts. Knockout of hisR in F. nucleatum results in a small but reproducible lag in growth compared to WT during exponential phase, suggesting that HisR may play a role in growth of this periodontal pathogen. | ||
| - | + | Discovery, characterization, and structure of a cofactor-independent histidine racemase from the oral pathogen Fusobacterium nucleatum.,Lamer T, Chen P, Venter MJ, van Belkum MJ, Wijewardane A, Wu C, Lemieux MJ, Vederas JC J Biol Chem. 2024 Nov;300(11):107896. doi: 10.1016/j.jbc.2024.107896. Epub 2024 , Oct 17. PMID:39424140<ref>PMID:39424140</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9cr6" style="background-color:#fffaf0;"></div> |
| - | [[Category: Chen | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Lemieux | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Fusobacterium nucleatum]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chen P]] | ||
| + | [[Category: Lamer T]] | ||
| + | [[Category: Lemieux MJ]] | ||
| + | [[Category: Vederas JC]] | ||
Current revision
Crystal structure of histidine racemase (HisR) of Fusobacterium nucleatum (C209S)
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