1v9l
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1v9l.jpg|left|200px]] | [[Image:1v9l.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1v9l", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1v9l| PDB=1v9l | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''L-glutamate dehydrogenase from Pyrobaculum islandicum complexed with NAD''' | '''L-glutamate dehydrogenase from Pyrobaculum islandicum complexed with NAD''' | ||
| Line 32: | Line 29: | ||
[[Category: Sakuraba, H.]] | [[Category: Sakuraba, H.]] | ||
[[Category: Tsuge, H.]] | [[Category: Tsuge, H.]] | ||
| - | [[Category: | + | [[Category: Protein-nad complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:15:59 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:16, 3 May 2008
L-glutamate dehydrogenase from Pyrobaculum islandicum complexed with NAD
Overview
The extremely thermostable NAD-dependent glutamate dehydrogenase (NAD-GluDH) from Pyrobaculum islandicum, a member of the Crenarchaeota, was crystallized, and its 3D structure has been determined by X-ray diffraction methods. The homohexameric structure of Pb. islandicum glutamate dehydrogenase (Pis-GluDH) was solved and refined at a resolution of 2.9A with a crystallographic R-factor of 19.9% (Rfree 26.0%). The structure indicates that each subunit consists of two domains separated by a deep cleft containing an active site. The secondary structural elements and catalytically important residues of the enzyme were highly conserved among the NAD(P)-dependent GluDHs from other sources. A structural comparison of Pis-GluDH with other NAD(P)-dependent GluDHs suggests that a significant difference in the alpha8-loop-alpha9 region of this enzyme is associated with its coenzyme specificity. From the analysis of the 3D structure, hydrophobic interactions between intersubunits were found to be important features for the enzyme oligomerization. It has been reported that Pis-GluDH is highly thermostable, like the GluDH of the hyperthermophilic archaeum Pyrococcus furiosus, and the increase in the intersubunit ion pair networks is responsible for the extreme thermostability of the Pc. furiosus enzyme. However, the number of intersubunit ion pairs in the Pis-GluDH molecules is much smaller than those of the Pc. furiosus GluDH. The number of hydrophobic interactions at the intersubunit interfaces were increased and responsible for the extremely high thermostability. This indicates that the major molecular strategy for high thermostability of the GluDHs may be different for each hyperthermophile.
About this Structure
1V9L is a Single protein structure of sequence from Pyrobaculum islandicum. Full crystallographic information is available from OCA.
Reference
The first crystal structure of hyperthermostable NAD-dependent glutamate dehydrogenase from Pyrobaculum islandicum., Bhuiya MW, Sakuraba H, Ohshima T, Imagawa T, Katunuma N, Tsuge H, J Mol Biol. 2005 Jan 14;345(2):325-37. PMID:15571725 Page seeded by OCA on Sat May 3 12:15:59 2008
