1v9p
From Proteopedia
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[[Image:1v9p.gif|left|200px]] | [[Image:1v9p.gif|left|200px]] | ||
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| - | | | + | {{STRUCTURE_1v9p| PDB=1v9p | SCENE= }} |
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'''Crystal Structure Of Nad+-Dependent DNA Ligase''' | '''Crystal Structure Of Nad+-Dependent DNA Ligase''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1V9P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_filiformis Thermus filiformis]. This structure supersedes the now removed PDB entry | + | 1V9P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_filiformis Thermus filiformis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1dgt 1dgt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9P OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of NAD(+)-dependent DNA ligase: modular architecture and functional implications., Lee JY, Chang C, Song HK, Moon J, Yang JK, Kim HK, Kwon ST, Suh SW, EMBO J. 2000 Mar 1;19(5):1119-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10698952 10698952] | Crystal structure of NAD(+)-dependent DNA ligase: modular architecture and functional implications., Lee JY, Chang C, Song HK, Moon J, Yang JK, Kim HK, Kwon ST, Suh SW, EMBO J. 2000 Mar 1;19(5):1119-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10698952 10698952] | ||
| - | [[Category: DNA ligase (NAD(+))]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus filiformis]] | [[Category: Thermus filiformis]] | ||
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[[Category: Suh, S W.]] | [[Category: Suh, S W.]] | ||
[[Category: Yang, J K.]] | [[Category: Yang, J K.]] | ||
| - | [[Category: | + | [[Category: Nad+-dependent dna ligase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:16:18 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:16, 3 May 2008
Crystal Structure Of Nad+-Dependent DNA Ligase
Overview
DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor. Despite the difference in cofactor specificity and limited overall sequence similarity, the two classes of DNA ligase share basically the same catalytic mechanism. In this study, the crystal structure of an NAD(+)-dependent DNA ligase from Thermus filiformis, a 667 residue multidomain protein, has been determined by the multiwavelength anomalous diffraction (MAD) method. It reveals highly modular architecture and a unique circular arrangement of its four distinct domains. It also provides clues for protein flexibility and DNA-binding sites. A model for the multidomain ligase action involving large conformational changes is proposed.
About this Structure
1V9P is a Single protein structure of sequence from Thermus filiformis. This structure supersedes the now removed PDB entry 1dgt. Full crystallographic information is available from OCA.
Reference
Crystal structure of NAD(+)-dependent DNA ligase: modular architecture and functional implications., Lee JY, Chang C, Song HK, Moon J, Yang JK, Kim HK, Kwon ST, Suh SW, EMBO J. 2000 Mar 1;19(5):1119-29. PMID:10698952 Page seeded by OCA on Sat May 3 12:16:18 2008
