This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ytv
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1ytv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ytv, resolution 1.80Å" /> '''Maltose-binding pro...)
Next diff →
Revision as of 18:18, 12 November 2007
|
Maltose-binding protein fusion to a C-terminal fragment of the V1a vasopressin receptor
Overview
The V1 vascular vasopressin receptor (V1R) is a G-protein-coupled receptor, (GPCR) involved in the regulation of body-fluid osmolality, blood volume, and blood pressure. Signal transduction is mediated by the third, intracellular loop of this seven-transmembrane protein as well as by the, C-terminal cytoplasmic segment. A chimera of the maltose-binding protein, (MBP) and the C-terminal segment of V1R has been cloned, expressed, purified and crystallized. The crystals belong to space group P2(1), with, unit-cell parameters a = 51.10, b = 66.56, c = 115.72 A, beta = 95.99, degrees. The 1.8 A crystal structure reveals the conformation of MBP and, part of the linker region of this chimera, with the C-terminal segment, being unstructured. This may reflect a conformational plasticity in the, C-terminal segment that may be necessary for proper function of V1R.
About this Structure
1YTV is a Protein complex structure of sequences from Escherichia coli and Homo sapiens with MAL as ligand. Full crystallographic information is available from OCA.
Reference
A C-terminal segment of the V1R vasopressin receptor is unstructured in the crystal structure of its chimera with the maltose-binding protein., Adikesavan NV, Mahmood SS, Stanley N, Xu Z, Wu N, Thibonnier M, Shoham M, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt, 4):341-5. Epub 2005 Mar 24. PMID:16511036
Page seeded by OCA on Mon Nov 12 20:24:33 2007
