1yuk

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Revision as of 18:18, 12 November 2007

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spinqualitylabelsall models 1yuk, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The crystal structure of the PSI/Hybrid domain/ I-EGF1 segment from the human integrin beta2 at 1.8 resolution

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Integrins are modular (alphabeta) heterodimeric proteins that mediate cell, adhesion and convey signals across the plasma membrane. Interdomain, motions play a key role in signal transduction by propagating structural, changes through the molecule, thus controlling the activation state and, adhesive properties of the integrin. We expressed a soluble fragment of, the human integrin beta2 subunit comprising the plexin-semaphorin-integrin, domain (PSI)/hybrid domain/I-EGF1 fragment and present its crystal, structure at 1.8-A resolution. The structure reveals an elongated molecule, with a rigid architecture stabilized by nine disulfide bridges. The PSI, domain is located centrally and participates in the formation of extended, interfaces with the hybrid domain and I-EGF1 domains, respectively. The, hybrid domain/PSI interface involves the burial of an Arg residue, and, contacts between PSI and I-EGF1 are mainly mediated by well conserved Arg, and Trp residues. Conservation of key interacting residues across the, various integrin beta subunits sequences suggests that our structure, represents a good model for the entire integrin family. Superposition with, the integrin beta3 receptor in its bent conformation suggests that an, articulation point is present at the linkage between its I-EGF1 and I-EGF2, modules and underlines the importance of this region for the control of, integrin-mediated cell adhesion.

Disease

Known disease associated with this structure: Leukocyte adhesion deficiency OMIM:[600065]

About this Structure

1YUK is a Protein complex structure of sequences from Homo sapiens with NDG as ligand. Full crystallographic information is available from OCA.

Reference

The crystal structure of the plexin-semaphorin-integrin domain/hybrid domain/I-EGF1 segment from the human integrin beta2 subunit at 1.8-A resolution., Shi M, Sundramurthy K, Liu B, Tan SM, Law SK, Lescar J, J Biol Chem. 2005 Aug 26;280(34):30586-93. Epub 2005 Jun 17. PMID:15965234

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