Sandbox Reserved 1845

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Revision as of 19:58, 27 March 2025

This Sandbox is Reserved from March 18 through September 1, 2025 for use in the course CH462 Biochemistry II taught by R. Jeremy Johnson and Mark Macbeth at the Butler University, Indianapolis, USA. This reservation includes Sandbox Reserved 1828 through Sandbox Reserved 1846.

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LCC Crystalized Structure of Leaf-branch compost cutinase

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1 Introduction
2 Structural Overview
- 2.1 Catalytic Triad
- 2.2 Ligand Binding Site
3 Mutations

Introduction

Structural Overview

Catalytic Triad

Ligand Binding Site

Figure 1

Mutations

F243

F243I

F243W

T96

T96M

 T96M is located within the core of the protein. Replacing Threonine with methionine, reduces polarity as well as increasing hydrophobicity. This change leads to an overall improvement in the packing of the hydrophobic core of the protein. This mutation stabilizes the enzyme's folding, which makes it more overall resistant to higher thermodynamic conditions.

Y127

Y127G

 Y127G is located in a loop near the surface of the protein. Replacing Tyrosine with a glycine increases flexibility. By replacing an aromatic bulky amino acid with the smallest amino acid, means that there is more flexibility in the loop.

N246

S283 & D238

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References

^[3]

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@@ Line 28: / Line 28: @@
 === T96 ===
 T96M
-  Located within the core of the protein. Replacing Threonine with methionine, reduces polarity as well as increasing hydrophobicity. This change leads to an overall improvement in the packing of the hydrophobic core of the protein.
+  T96M is located within the core of the protein. Replacing Threonine with methionine, reduces polarity as well as increasing hydrophobicity. This change leads to an overall improvement in the packing of the hydrophobic core of the protein. This mutation stabilizes the enzyme's folding, which makes it more overall resistant to higher thermodynamic conditions.
 === Y127 ===
+Y127G
+  Y127G is located in a loop near the surface of the protein. Replacing Tyrosine with a glycine increases flexibility. By replacing an aromatic bulky amino acid with the smallest amino acid, means that there is more flexibility in the loop.
 === N246 ===