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1vf8
From Proteopedia
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[[Image:1vf8.gif|left|200px]] | [[Image:1vf8.gif|left|200px]] | ||
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'''The Crystal Structure of Ym1 at 1.31 A Resolution''' | '''The Crystal Structure of Ym1 at 1.31 A Resolution''' | ||
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[[Category: Liaw, S H.]] | [[Category: Liaw, S H.]] | ||
[[Category: Tsai, M L.]] | [[Category: Tsai, M L.]] | ||
| - | [[Category: | + | [[Category: Chi-lectin]] |
| - | [[Category: | + | [[Category: Chitinase]] |
| - | [[Category: | + | [[Category: Functional versatility]] |
| - | [[Category: | + | [[Category: Structural plasticity]] |
| - | [[Category: | + | [[Category: Ym1]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:28:18 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:28, 3 May 2008
The Crystal Structure of Ym1 at 1.31 A Resolution
Overview
Upon nematode infection, murine peritoneal macrophages synthesize and secrete large amounts of the Ym1 protein, which is a unique functional marker for alternatively activated macrophages in T(H)2-mediated inflammatory responses. Ym1 shares significant structural similarity to the family 18 chitinases. Previously, Ym1 has been studied with respect to its carbohydrate-binding ability and glycosyl hydrolysis activity and this has led to various inconclusive interpretations. Our present co-crystallization and soaking experiments with various glucosamine or N-acetylglucosamine oligomers yield only the uncomplexed Ym1. The refined Ym1 structure at 1.31A resolution clearly displays a water cluster forming an extensive hydrogen bond network with the "active-site" residues. This water cluster contributes notable electron density to lower resolution maps and this might have misled and given rise to a previous proposal for a monoglucosamine-binding site for Ym1. A structural comparison of family 18 glycosidase (-like) proteins reveals a lack of several conserved residues in Ym1, and illustrates the versatility of the divergent active sites. Therefore, Ym1 may lack N-acetylglucosamine-binding affinity, and this suggests that a new direction should be taken to unravel the function of Ym1.
About this Structure
1VF8 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The crystal structure of Ym1 at 1.31 A resolution., Tsai ML, Liaw SH, Chang NC, J Struct Biol. 2004 Dec;148(3):290-6. PMID:15522777 Page seeded by OCA on Sat May 3 12:28:18 2008
