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| | ==Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy and DEER== | | ==Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy and DEER== |
| - | <StructureSection load='5uk6' size='340' side='right'caption='[[5uk6]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='5uk6' size='340' side='right'caption='[[5uk6]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5uk6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UK6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5uk6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UK6 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR, 10 models</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">alr3165 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uk6 OCA], [http://pdbe.org/5uk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uk6 RCSB], [http://www.ebi.ac.uk/pdbsum/5uk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uk6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uk6 OCA], [https://pdbe.org/5uk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uk6 RCSB], [https://www.ebi.ac.uk/pdbsum/5uk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uk6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8YSC4_NOSS1 Q8YSC4_NOSS1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anabaena 7120]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Brown, L S]] | + | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] |
| - | [[Category: Donohue, M]] | + | [[Category: Brown LS]] |
| - | [[Category: Ladizhansky, V]] | + | [[Category: Donohue M]] |
| - | [[Category: Milikisiyants, S]] | + | [[Category: Ladizhansky V]] |
| - | [[Category: Munro, R A]] | + | [[Category: Milikisiyants S]] |
| - | [[Category: Smirnov, A I]] | + | [[Category: Munro RA]] |
| - | [[Category: Smirnova, T I]] | + | [[Category: Smirnov AI]] |
| - | [[Category: Wang, S]] | + | [[Category: Smirnova TI]] |
| - | [[Category: Ward, M E]] | + | [[Category: Wang S]] |
| - | [[Category: Anabeana sensory rhodopsin]]
| + | [[Category: Ward ME]] |
| - | [[Category: Seven-transmembrane protein]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Trimer]]
| + | |
| Structural highlights
Function
Q8YSC4_NOSS1
Publication Abstract from PubMed
Oligomerization of membrane proteins is common in nature. Here, we combine spin-labeling double electron-electron resonance (DEER) and solid-state NMR (ssNMR) spectroscopy to refine the structure of an oligomeric integral membrane protein, Anabaena sensory rhodopsin (ASR), reconstituted in a lipid environment. An essential feature of such a combined approach is that it provides structural distance restraints spanning a range of ca 3-60A while using the same sample preparation (i.e., mutations, paramagnetic labeling, and reconstitution in lipid bilayers) for both ssNMR and DEER. Direct modeling of the multispin effects on DEER signal allowed for the determination of the oligomeric order and for obtaining long-range DEER distance restraints between the ASR trimer subunits that were used to refine the ssNMR structure of ASR. The improved structure of the ASR trimer revealed a more compact packing of helices and side chains at the intermonomer interface, compared to the structure determined using the ssNMR data alone. The extent of the refinement is significant when compared with typical helix movements observed for the active states of homologous proteins. Our combined approach of using complementary DEER and NMR measurements for the determination of oligomeric structures would be widely applicable to membrane proteins where paramagnetic tags can be introduced. Such a method could be used to study the effects of the lipid membrane composition on protein oligomerization and to observe structural changes in protein oligomers upon drug, substrate, and co-factor binding.
Oligomeric Structure of Anabaena Sensory Rhodopsin in a Lipid Bilayer Environment by Combining Solid-State NMR and Long-range DEER Constraints.,Milikisiyants S, Wang S, Munro RA, Donohue M, Ward ME, Bolton D, Brown LS, Smirnova TI, Ladizhansky V, Smirnov AI J Mol Biol. 2017 Jun 16;429(12):1903-1920. doi: 10.1016/j.jmb.2017.05.005. Epub, 2017 May 10. PMID:28501588[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Milikisiyants S, Wang S, Munro RA, Donohue M, Ward ME, Bolton D, Brown LS, Smirnova TI, Ladizhansky V, Smirnov AI. Oligomeric Structure of Anabaena Sensory Rhodopsin in a Lipid Bilayer Environment by Combining Solid-State NMR and Long-range DEER Constraints. J Mol Biol. 2017 Jun 16;429(12):1903-1920. doi: 10.1016/j.jmb.2017.05.005. Epub, 2017 May 10. PMID:28501588 doi:http://dx.doi.org/10.1016/j.jmb.2017.05.005
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