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| | <StructureSection load='6ndt' size='340' side='right'caption='[[6ndt]], [[Resolution|resolution]] 1.42Å' scene=''> | | <StructureSection load='6ndt' size='340' side='right'caption='[[6ndt]], [[Resolution|resolution]] 1.42Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ndt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"spirillum_dentium"_(miller_1889)_sternberg_1892 "spirillum dentium" (miller 1889) sternberg 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NDT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ndt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_denticola Treponema denticola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NDT FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.424Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flgE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158 "Spirillum dentium" (Miller 1889) Sternberg 1892])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ndt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ndt OCA], [http://pdbe.org/6ndt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ndt RCSB], [http://www.ebi.ac.uk/pdbsum/6ndt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ndt ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ndt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ndt OCA], [https://pdbe.org/6ndt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ndt RCSB], [https://www.ebi.ac.uk/pdbsum/6ndt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ndt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9RQB6_TREDN Q9RQB6_TREDN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6ndt" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6ndt" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Flagellar protein 3D structures|Flagellar protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Crane, B R]] | + | [[Category: Treponema denticola]] |
| - | [[Category: Lynch, M J]] | + | [[Category: Crane BR]] |
| - | [[Category: Crosslinking]] | + | [[Category: Lynch MJ]] |
| - | [[Category: Dehydroalanine]]
| + | |
| - | [[Category: Flge]]
| + | |
| - | [[Category: Hook]]
| + | |
| - | [[Category: Lysinoalanine]]
| + | |
| - | [[Category: Motor protein]]
| + | |
| - | [[Category: Periodontal disease]]
| + | |
| - | [[Category: Spirochete]]
| + | |
| Structural highlights
Function
Q9RQB6_TREDN
Publication Abstract from PubMed
The flagellar hook protein FlgE from spirochaete bacteria self-catalyzes the formation of an unusual inter-subunit lysinoalanine (Lal) crosslink that is critical for cell motility. Unlike other known examples of Lal biosynthesis, conserved cysteine and lysine residues in FlgE spontaneously react to form Lal without the involvement of additional enzymes. Oligomerization of FlgE via its D0 and Dc domains drives assembly of the crosslinking site at the D1-D2 domain interface. Structures of the FlgED2 domain, dehydroalanine (DHA) intermediate and Lal crosslinked FlgE subunits reveal successive snapshots of the reaction. Cys178 flips from a buried configuration to release hydrogen sulfide (H2S/HS(-)) and produce DHA. Interface residues provide hydrogen bonds to anchor the active site, facilitate beta-elimination of Cys178 and polarize the peptide backbone to activate DHA for reaction with Lys165. Cysteine-reactive molecules accelerate DHA formation, whereas nucleophiles can intercept the DHA intermediate, thereby indicating a potential for Lal crosslink inhibitors to combat spirochaetal diseases.
Structure and chemistry of lysinoalanine crosslinking in the spirochaete flagella hook.,Lynch MJ, Miller M, James M, Zhang S, Zhang K, Li C, Charon NW, Crane BR Nat Chem Biol. 2019 Aug 12. pii: 10.1038/s41589-019-0341-3. doi:, 10.1038/s41589-019-0341-3. PMID:31406373[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lynch MJ, Miller M, James M, Zhang S, Zhang K, Li C, Charon NW, Crane BR. Structure and chemistry of lysinoalanine crosslinking in the spirochaete flagella hook. Nat Chem Biol. 2019 Aug 12. pii: 10.1038/s41589-019-0341-3. doi:, 10.1038/s41589-019-0341-3. PMID:31406373 doi:http://dx.doi.org/10.1038/s41589-019-0341-3
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