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1yyb
From Proteopedia
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(New page: 200px<br /> <applet load="1yyb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yyb" /> '''Solution structure of 1-26 fragment of huma...)
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Revision as of 18:20, 12 November 2007
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Solution structure of 1-26 fragment of human programmed cell death 5 protein
Overview
PDCD5-(1-26) is a N-terminal 26-residue fragment of human PDCD5, (programmed cell death 5) protein. PDCD5 is an important novel protein, that regulates both apoptotic and non-apoptotic programmed cell death. The, conformation of PDCD5 protein is a stable helical core consisting of a, triple-helix bundle and two dissociated terminal regions. The N-terminal, region is ordered and contains abundant secondary structure., Overexpression and purification of the N-terminal 26-residure fragment, PDCD5-(1-26), was performed in this study to better understand its, tertiary structure. The spectroscopic studies using CD and hetero- and, homo-nuclear NMR methods determine a stable alpha-helix formed by, Asp3-Ala19 of PDCD5-(1-26). The N-terminal residues Asp3-Ala19 of PDCD5, were then affirmed to have the capacity to form a stable alpha-helix, independently of the core of the protein. Analysis of the helical peptide, of PDCD5-(1-26) indicates that the surface of this well-formed alpha-helix, has a unique electrostatic potential character. This may provide an, environment for the N-terminal alpha-helix of PDCD5 to serve as an, independent functional entity of the protein. The apoptosis activity assay, shows that the deletion of the N-terminal alpha-helix of PDCD5, significantly attenuates the apoptosis-promoting effects on HL-60 cells, induced by serum withdrawal.
About this Structure
1YYB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The N-terminal 26-residue fragment of human programmed cell death 5 protein can form a stable alpha-helix having unique electrostatic potential character., Liu D, Yao H, Chen Y, Feng Y, Chen Y, Wang J, Biochem J. 2005 Nov 15;392(Pt 1):47-54. PMID:16083422
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