6qsu
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Helicobacter pylori urease with BME bound in the active site== |
| - | <StructureSection load='6qsu' size='340' side='right'caption='[[6qsu]]' scene=''> | + | <StructureSection load='6qsu' size='340' side='right'caption='[[6qsu]], [[Resolution|resolution]] 2.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6qsu]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QSU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QSU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qsu OCA], [https://pdbe.org/6qsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qsu RCSB], [https://www.ebi.ac.uk/pdbsum/6qsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qsu ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.4Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qsu OCA], [https://pdbe.org/6qsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qsu RCSB], [https://www.ebi.ac.uk/pdbsum/6qsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qsu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/URE23_HELPY URE23_HELPY] Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.<ref>PMID:8039935</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Infection of the human stomach by Helicobacter pylori remains a worldwide problem and greatly contributes to peptic ulcer disease and gastric cancer. Without active intervention approximately 50% of the world population will continue to be infected with this gastric pathogen. Current eradication, called triple therapy, entails a proton-pump inhibitor and two broadband antibiotics, however resistance to either clarithromycin or metronidazole is greater than 25% and rising. Therefore, there is an urgent need for a targeted, high-specificity eradication drug. Gastric infection by H. pylori depends on the expression of a nickel-dependent urease in the cytoplasm of the bacteria. Here, we report the 2.0 A resolution structure of the 1.1 MDa urease in complex with an inhibitor by cryo-electron microscopy and compare it to a beta-mercaptoethanol-inhibited structure at 2.5 A resolution. The structural information is of sufficient detail to aid in the development of inhibitors with high specificity and affinity. | ||
| + | |||
| + | Cryo-EM structure of Helicobacter pylori urease with an inhibitor in the active site at 2.0 A resolution.,Cunha ES, Chen X, Sanz-Gaitero M, Mills DJ, Luecke H Nat Commun. 2021 Jan 11;12(1):230. doi: 10.1038/s41467-020-20485-6. PMID:33431861<ref>PMID:33431861</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6qsu" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Urease 3D structures|Urease 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Helicobacter pylori]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Cunha E]] |
| + | [[Category: Luecke H]] | ||
Current revision
Helicobacter pylori urease with BME bound in the active site
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