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| | <SX load='6s6s' size='340' side='right' viewer='molstar' caption='[[6s6s]], [[Resolution|resolution]] 3.90Å' scene=''> | | <SX load='6s6s' size='340' side='right' viewer='molstar' caption='[[6s6s]], [[Resolution|resolution]] 3.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6s6s]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29145 Atcc 29145]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S6S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S6S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6s6s]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Azospirillum_brasilense Azospirillum brasilense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S6S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gltB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192 ATCC 29145]), gltD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192 ATCC 29145])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_synthase_(NADPH) Glutamate synthase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.13 1.4.1.13] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s6s OCA], [https://pdbe.org/6s6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s6s RCSB], [https://www.ebi.ac.uk/pdbsum/6s6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s6s ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s6s OCA], [http://pdbe.org/6s6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s6s RCSB], [http://www.ebi.ac.uk/pdbsum/6s6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s6s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GLTB_AZOBR GLTB_AZOBR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6s6s" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6s6s" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Glutamate synthase|Glutamate synthase]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Atcc 29145]] | + | [[Category: Azospirillum brasilense]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bolognesi, M]] | + | [[Category: Bolognesi M]] |
| - | [[Category: Chaves-Sanjuan, A]] | + | [[Category: Chaves-Sanjuan A]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Flavoprotein]]
| + | |
| - | [[Category: Glutamate synthesy]]
| + | |
| - | [[Category: Oligomeric assembly]]
| + | |
| Structural highlights
Function
GLTB_AZOBR
Publication Abstract from PubMed
Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an alpha-subunit (alphaGltS) and a beta-subunit (betaGltS) that assemble in different alphabeta oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (alpha4beta3, alpha4beta4, alpha6beta4 and alpha6beta6, in the 3.5- to 4.1-A resolution range). Our study provides a comprehensive GltS model that details the inter-protomeric assemblies and allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S](+1,+2) clusters within betaGltS.
Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.,Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M J Mol Biol. 2019 Aug 29. pii: S0022-2836(19)30520-0. doi:, 10.1016/j.jmb.2019.08.011. PMID:31473159[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M. Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies. J Mol Biol. 2019 Aug 29. pii: S0022-2836(19)30520-0. doi:, 10.1016/j.jmb.2019.08.011. PMID:31473159 doi:http://dx.doi.org/10.1016/j.jmb.2019.08.011
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