7bc4
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Cryo-EM structure of fatty acid synthase (FAS) from Pichia pastoris== |
| - | <StructureSection load='7bc4' size='340' side='right'caption='[[7bc4]]' scene=''> | + | <StructureSection load='7bc4' size='340' side='right'caption='[[7bc4]], [[Resolution|resolution]] 3.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bc4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_phaffii_GS115 Komagataella phaffii GS115]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BC4 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bc4 OCA], [https://pdbe.org/7bc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bc4 RCSB], [https://www.ebi.ac.uk/pdbsum/7bc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bc4 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bc4 OCA], [https://pdbe.org/7bc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bc4 RCSB], [https://www.ebi.ac.uk/pdbsum/7bc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bc4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C4QY10_KOMPG C4QY10_KOMPG] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type I fatty acid synthases (FASs) are critical metabolic enzymes which are common targets for bioengineering in the production of biofuels and other products. Serendipitously, we identified FAS as a contaminant in a cryoEM dataset of virus-like particles (VLPs) purified from P. pastoris, an important model organism and common expression system used in protein production. From these data, we determined the structure of P. pastoris FAS to 3.1 A resolution. While the overall organisation of the complex was typical of type I FASs, we identified several differences in both structural and enzymatic domains through comparison with the prototypical yeast FAS from S. cerevisiae. Using focussed classification, we were also able to resolve and model the mobile acyl-carrier protein (ACP) domain, which is key for function. Ultimately, the structure reported here will be a useful resource for further efforts to engineer yeast FAS for synthesis of alternate products. | ||
| + | |||
| + | Structural insight into Pichia pastoris fatty acid synthase.,Snowden JS, Alzahrani J, Sherry L, Stacey M, Rowlands DJ, Ranson NA, Stonehouse NJ Sci Rep. 2021 May 7;11(1):9773. doi: 10.1038/s41598-021-89196-2. PMID:33963233<ref>PMID:33963233</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bc4" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Fatty acid synthase 3D structures|Fatty acid synthase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Komagataella phaffii GS115]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Alzahrani J]] |
| + | [[Category: Ranson NA]] | ||
| + | [[Category: Rowlands DJ]] | ||
| + | [[Category: Sherry L]] | ||
| + | [[Category: Snowden JS]] | ||
| + | [[Category: Stacey M]] | ||
| + | [[Category: Stonehouse NJ]] | ||
Current revision
Cryo-EM structure of fatty acid synthase (FAS) from Pichia pastoris
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