1vj7

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1vj7.gif|left|200px]]
[[Image:1vj7.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1vj7 |SIZE=350|CAPTION= <scene name='initialview01'>1vj7</scene>, resolution 2.10&Aring;
+
The line below this paragraph, containing "STRUCTURE_1vj7", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GPX:GUANOSINE+5&#39;-DIPHOSPHATE+2&#39;&#58;3&#39;-CYCLIC+MONOPHOSPHATE'>GPX</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= RELA, REL, SPOT, RSH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119602 Streptococcus dysgalactiae subsp. equisimilis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1vj7| PDB=1vj7 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vj7 OCA], [http://www.ebi.ac.uk/pdbsum/1vj7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vj7 RCSB]</span>
+
-
}}
+
'''Crystal structure of the bifunctional catalytic fragment of RelSeq, the RelA/SpoT homolog from Streptococcus equisimilis.'''
'''Crystal structure of the bifunctional catalytic fragment of RelSeq, the RelA/SpoT homolog from Streptococcus equisimilis.'''
Line 30: Line 27:
[[Category: Malke, H.]]
[[Category: Malke, H.]]
[[Category: Mechold, U.]]
[[Category: Mechold, U.]]
-
[[Category: (p)ppgpp]]
+
[[Category: Alpha beta 2-layer sandwich]]
-
[[Category: alpha beta 2-layer sandwich]]
+
[[Category: Gdp]]
-
[[Category: gdp]]
+
[[Category: Hd domain]]
-
[[Category: hd domain]]
+
[[Category: Helix bundle]]
-
[[Category: helix bundle]]
+
[[Category: Magic spot]]
-
[[Category: magic spot]]
+
[[Category: Manganese]]
-
[[Category: manganese]]
+
[[Category: Ppg2':3'p]]
-
[[Category: ppg2':3'p]]
+
[[Category: Ppgpp]]
-
[[Category: ppgpp]]
+
[[Category: Rela]]
-
[[Category: rela]]
+
[[Category: Rsh]]
-
[[Category: rsh]]
+
[[Category: Spot]]
-
[[Category: spot]]
+
[[Category: Stringent effector]]
-
[[Category: stringent effector]]
+
[[Category: Stringent factor]]
-
[[Category: stringent factor]]
+
[[Category: Stringent response]]
-
[[Category: stringent response]]
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:35:25 2008''
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:25:13 2008''
+

Revision as of 09:35, 3 May 2008

Image:1vj7.gif

Template:STRUCTURE 1vj7

Crystal structure of the bifunctional catalytic fragment of RelSeq, the RelA/SpoT homolog from Streptococcus equisimilis.


Overview

Enzymes of the Rel/Spo family enable bacteria to survive prolonged periods of nutrient limitation by producing an intracellular signaling alarmone, (p)ppGpp, which triggers the so-called stringent response. Both the synthesis of (p)ppGpp from ATP and GDP(GTP), and its hydrolysis to GDP(GTP) and pyrophosphate, are catalyzed by Rel/Spo proteins. The 2.1 A crystal structure of the bifunctional catalytic fragment of the Rel/Spo homolog from Streptococcus dysgalactiae subsp. equisimilis, Rel(Seq), reveals two conformations of the enzyme corresponding to known reciprocal activity states: (p)ppGpp-hydrolase-OFF/(p)ppGpp-synthetase-ON and hydrolase-ON/synthetase-OFF. The hydrolase and synthetase domains bear remarkable similarities to the catalytic domains of the cyclic phosphodiesterase and nucleotidyltransferase superfamilies, respectively. The active sites, separated by more than 30 A, contain bound nucleotides including an unusual (p)ppGpp derivative, GDP-2':3'-cyclic monophosphate. Reciprocal regulation of the antagonistic catalytic activities, suggested by the structure, is supported by mutagenesis experiments and appears to involve ligand-induced signal transmission between the two active sites.

About this Structure

1VJ7 is a Single protein structure of sequence from Streptococcus dysgalactiae subsp. equisimilis. Full crystallographic information is available from OCA.

Reference

Conformational antagonism between opposing active sites in a bifunctional RelA/SpoT homolog modulates (p)ppGpp metabolism during the stringent response [corrected]., Hogg T, Mechold U, Malke H, Cashel M, Hilgenfeld R, Cell. 2004 Apr 2;117(1):57-68. PMID:15066282 Page seeded by OCA on Sat May 3 12:35:25 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1vj7"
Personal tools