9kas
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of anti-sulfonylurea antibody scFv in complex with chlorpropamide== | |
| + | <StructureSection load='9kas' size='340' side='right'caption='[[9kas]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9kas]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KAS FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.401Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1EEY:1-(4-chlorophenyl)sulfonyl-3-propyl-urea'>A1EEY</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kas OCA], [https://pdbe.org/9kas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kas RCSB], [https://www.ebi.ac.uk/pdbsum/9kas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kas ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The limited understanding of the broad-specific antibody recognition mechanism significantly hinders the development of immunoassays for simultaneously detecting illegal adulterants. Herein, a recombinant antisulfonylureas (SUs) single-chain variable fragment (scFv), which retained the properties of its parental monoclonal antibody, was successfully generated. X-ray crystallography, molecular docking, functional assays, and mutation validation were used to investigate the structure-function relationships underlying antibody-SUs binding. Our study revealed three key mechanisms for broad specificity: (1) the conformational adaptability of the scFv, which enabled various SUs to access the binding pocket; (2) the role of the Trp98 residue in CDR-L3 in modulating binding affinities among multiple SUs; and (3) the design of haptens with common structures and more rigid R substituents, which emerged as a promising strategy for generating broad-specific antibodies. This study provides a comprehensive analysis of the broad-specific recognition mechanism, offering valuable insights for rational hapten design and targeted antibody evolution to advance multitarget immunoassays. | ||
| - | + | Structure Profiling of Broad-Specificity Immunoassays: Multitarget Recognition for Sulfonylurea Adulteration in Food.,Yu X, Zhou T, Pan K, Fan MH, Yan X, Huang XA, Zhang H, Shen X, Xie H, Gao YG, Lei H J Agric Food Chem. 2025 Apr 16;73(15):9348-9358. doi: 10.1021/acs.jafc.5c00655. , Epub 2025 Apr 2. PMID:40173361<ref>PMID:40173361</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9kas" style="background-color:#fffaf0;"></div> |
| - | [[Category: Lei | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Gao YG]] | ||
| + | [[Category: Lei HT]] | ||
| + | [[Category: Yu XT]] | ||
Current revision
Crystal structure of anti-sulfonylurea antibody scFv in complex with chlorpropamide
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Categories: Large Structures | Mus musculus | Gao YG | Lei HT | Yu XT
