1vkj

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[[Image:1vkj.gif|left|200px]]
[[Image:1vkj.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1vkj |SIZE=350|CAPTION= <scene name='initialview01'>1vkj</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1vkj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3&#39;-5&#39;-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1vkj| PDB=1vkj | SCENE= }}
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|RELATEDENTRY=[[1nst|1NST]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vkj OCA], [http://www.ebi.ac.uk/pdbsum/1vkj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vkj RCSB]</span>
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}}
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'''Crystal structure of heparan sulfate 3-O-sulfotransferase isoform 1 in the presence of PAP'''
'''Crystal structure of heparan sulfate 3-O-sulfotransferase isoform 1 in the presence of PAP'''
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==About this Structure==
==About this Structure==
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1VKJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry 1S6T. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VKJ OCA].
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1VKJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1s6t 1s6t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VKJ OCA].
==Reference==
==Reference==
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: [Heparan sulfate]-glucosamine 3-sulfotransferase 1]]
 
[[Category: Lee, K A.]]
[[Category: Lee, K A.]]
[[Category: Linhardt, R J.]]
[[Category: Linhardt, R J.]]
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[[Category: Pedersen, L C.]]
[[Category: Pedersen, L C.]]
[[Category: Thorp, S.]]
[[Category: Thorp, S.]]
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[[Category: heparan sulfate]]
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[[Category: Heparan sulfate]]
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[[Category: pap]]
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[[Category: Pap]]
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[[Category: papss]]
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[[Category: Papss]]
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[[Category: sulfotransferase]]
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[[Category: Sulfotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:38:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:25:42 2008''
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Revision as of 09:38, 3 May 2008

Image:1vkj.gif

Template:STRUCTURE 1vkj

Crystal structure of heparan sulfate 3-O-sulfotransferase isoform 1 in the presence of PAP


Overview

Heparan sulfate interacts with antithrombin, a protease inhibitor, to regulate blood coagulation. Heparan sulfate 3-O-sulfotransferase isoform 1 performs the crucial last step modification in the biosynthesis of anticoagulant heparan sulfate. This enzyme transfers the sulfuryl group (SO(3)) from 3'-phosphoadenosine 5'-phosphosulfate to the 3-OH position of a glucosamine residue to form the 3-O-sulfo glucosamine, a structural motif critical for binding of heparan sulfate to antithrombin. In this study, we report the crystal structure of 3-O-sulfotransferase isoform 1 at 2.5-A resolution in a binary complex with 3'-phosphoadenosine 5'-phosphate. This structure reveals residues critical for 3'-phosphoadenosine 5'-phosphosulfate binding and suggests residues required for the binding of heparan sulfate. In addition, site-directed mutagenesis analyses suggest that residues Arg-67, Lys-68, Arg-72, Glu-90, His-92, Asp-95, Lys-123, and Arg-276 are essential for enzymatic activity. Among these essential amino acid residues, we find that residues Arg-67, Arg-72, His-92, and Asp-95 are conserved in heparan sulfate 3-O-sulfotransferases but not in heparan N-deacetylase/N-sulfotransferase, suggesting a role for these residues in conferring substrate specificity. Results from this study provide information essential for understanding the biosynthesis of anticoagulant heparan sulfate and the general mechanism of action of heparan sulfate sulfotransferases.

About this Structure

1VKJ is a Single protein structure of sequence from Mus musculus. This structure supersedes the now removed PDB entry 1s6t. Full crystallographic information is available from OCA.

Reference

Crystal structure and mutational analysis of heparan sulfate 3-O-sulfotransferase isoform 1., Edavettal SC, Lee KA, Negishi M, Linhardt RJ, Liu J, Pedersen LC, J Biol Chem. 2004 Jun 11;279(24):25789-97. Epub 2004 Apr 1. PMID:15060080 Page seeded by OCA on Sat May 3 12:38:40 2008

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