1z5w

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Revision as of 18:23, 12 November 2007

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spinqualitylabelsall models 1z5w, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of gamma-tubulin bound to GTP

Overview

Microtubules are hollow polymers of alphabeta-tubulin that show, GTP-dependent assembly dynamics and comprise a critical part of the, eukaryotic cytoskeleton. Initiation of new microtubules in vivo requires, gamma-tubulin, organized as an oligomer within the 2.2-MDa gamma-tubulin, ring complex (gamma-TuRC) of higher eukaryotes. Structural insight is, lacking regarding gamma-tubulin, its oligomerization and how it promotes, microtubule assembly. Here we report the 2.7-A crystal structure of human, gamma-tubulin bound to GTP-gammaS (a non-hydrolysable GTP analogue). We, observe a 'curved' conformation for gamma-tubulin-GTPgammaS, similar to, that seen for GDP-bound, unpolymerized alphabeta-tubulin. Tubulins are, thought to represent a distinct class of GTP-binding proteins, and, conformational switching in gamma-tubulin might differ from the, nucleotide-dependent switching of signalling GTPases. A crystal packing, interaction replicates the lateral contacts between alpha- and, beta-tubulins in the microtubule, and this association probably forms the, basis for gamma-tubulin oligomerization within the gamma-TuRC. Laterally, associated gamma-tubulins in the gamma-TuRC might promote microtubule, nucleation by providing a template that enhances the intrinsically weak, lateral interaction between alphabeta-tubulin heterodimers. Because they, are dimeric, alphabeta-tubulins cannot form microtubule-like lateral, associations in the curved conformation. The lateral array of, gamma-tubulins we observe in the crystal reveals a unique functional, property of a monomeric tubulin.

About this Structure

1Z5W is a Single protein structure of sequence from Homo sapiens with MG and GTP as ligands. Full crystallographic information is available from OCA.

Reference

Insights into microtubule nucleation from the crystal structure of human gamma-tubulin., Aldaz H, Rice LM, Stearns T, Agard DA, Nature. 2005 May 26;435(7041):523-7. PMID:15917813

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