1aq8
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(New page: 200px<br /> <applet load="1aq8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aq8, resolution 2.0Å" /> '''STRUCTURE OF ALCALIG...)
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Revision as of 16:36, 29 October 2007
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STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE REDUCED WITH ASCORBATE
Overview
The structures of oxidized, reduced, nitrite-soaked oxidized and, nitrite-soaked reduced nitrite reductase from Alcaligenes faecalis have, been determined at 1.8-2.0 A resolution using data collected at -160, degrees C. The active site at cryogenic temperature, as at room, temperature, contains a tetrahedral type II copper site liganded by three, histidines and a water molecule. The solvent site is empty when crystals, are reduced with ascorbate. A fully occupied oxygen-coordinate nitrite, occupies the solvent site in crystals soaked in nitrite. Ascorbate-reduced, crystals soaked in a glycerol-methanol solution and nitrite at -40 degrees, C remain colorless at -160 degrees C but turn amber-brown when warmed, suggesting that NO is released. Nitrite is found at one-half occupancy., Five new ... [(full description)]
About this Structure
1AQ8 is a [Single protein] structure of sequence from [Alcaligenes faecalis] with CU as [ligand]. Active as [[1]], with EC number [1.7.99.3]. Full crystallographic information is available from [OCA].
Reference
Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. Mechanistic implications., Murphy ME, Turley S, Adman ET, J Biol Chem. 1997 Nov 7;272(45):28455-60. PMID:9353305
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