9j9j
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Nitrophenol monooxygenase RsPNPA from Rhodococcus sp. 21391== | |
| + | <StructureSection load='9j9j' size='340' side='right'caption='[[9j9j]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9j9j]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Unclassified_Rhodococcus Unclassified Rhodococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9J9J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9J9J FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9j9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9j9j OCA], [https://pdbe.org/9j9j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9j9j RCSB], [https://www.ebi.ac.uk/pdbsum/9j9j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9j9j ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NPCA_RHOOP NPCA_RHOOP] Involved in the degradation of para-nitrophenol (4-NP). Catalyzes both the initial hydroxylation of 4-NP to produce 4-nitrocatechol (4-NCA) and the subsequent oxidative release of the nitro group from 4-NCA to produce 2-hydroxy-1,4-benzoquinone. It can also use 4-nitroresorcinol as substrate with a rate of nitrite release similar to that observed with the two physiological substrates, 4-PN and 4-NCA.<ref>PMID:15262926</ref> <ref>PMID:9647818</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | BACKGROUND: Bioremediation relying on highly efficient degrading bacteria constitutes a promising and sustainable avenue for controlling and reducing nitrophenol contamination in the environment. A thorough understanding of the bacterial degradation mechanism of nitrophenol is of paramount importance for supporting the development of efficient microbial remediation technology. RESULTS: In this study, a new bacterium, Rhodococcus sp. 21391, endowed with superior p-nitrophenol (PNP) degradation ability was obtained. Genomic and comparative proteomic analyses revealed that it utilizes the 1,2,4-benzenetriol (BT) pathway for PNP degradation. The catalytic properties of the two-component p-nitrophenol monooxygenase RsNcpAB from the strain were investigated in vitro. The enzyme exhibited a broad substrate selectivity, catalyzing the oxidation of various nitrophenols and halogenated phenols, with significant potential for further research and development. Additionally, the crystal structure of the oxidative component of p-nitrophenol monooxygenase, RsNcpA, was determined. Structural analysis and site-directed mutagenesis revealed that residues Arg100 and His293 in the active site play a crucial role in enzyme catalysis, and a catalytic mechanism model was subsequently proposed. CONCLUSIONS: This study reports a high-performance nitrophenol-degrading bacterium and enzyme, and reveals their mechanisms at the molecular level. These findings increase the understanding of the bacterial degradation of nitrophenol, thereby providing a crucial foundation for the development of efficient bioremediation technologies. | ||
| - | + | Biodegradation of p-nitrophenol by Rhodococcus sp. 21391 unveils a two-component p-nitrophenol monooxygenase with broad substrate specificity.,Yang J, Lin S, Li W, Wang X, Li R Microb Cell Fact. 2025 Apr 17;24(1):85. doi: 10.1186/s12934-025-02712-1. PMID:40247276<ref>PMID:40247276</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9j9j" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Long | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Unclassified Rhodococcus]] | ||
| + | [[Category: Li R]] | ||
| + | [[Category: Lin S]] | ||
| + | [[Category: Long L]] | ||
| + | [[Category: Yang J]] | ||
Current revision
Nitrophenol monooxygenase RsPNPA from Rhodococcus sp. 21391
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