1vpw

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[[Image:1vpw.gif|left|200px]]
[[Image:1vpw.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1vpw", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>
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{{STRUCTURE_1vpw| PDB=1vpw | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vpw OCA], [http://www.ebi.ac.uk/pdbsum/1vpw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vpw RCSB]</span>
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'''STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA'''
'''STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA'''
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[[Category: Lu, F.]]
[[Category: Lu, F.]]
[[Category: Zalkin, H.]]
[[Category: Zalkin, H.]]
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[[Category: complex (dna-binding protein/dna)]]
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[[Category: Dna-binding regulatory protein]]
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[[Category: dna-binding regulatory protein]]
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[[Category: Minor groove bending]]
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[[Category: minor groove bending]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:46:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:27:07 2008''
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Revision as of 09:46, 3 May 2008

Image:1vpw.gif

Template:STRUCTURE 1vpw

STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA


Overview

The crystal structure of the purine repressor mutant L54M bound to hypoxanthine and to the purF operator provides a stereochemical understanding of the high DNA affinity of this hinge helix mutant. Comparison of the PurR L54M-DNA complex to that of the wild type PurR-DNA complex reveals that these purine repressors bind and kink DNA similarly despite significant differences in their minor groove contacts and routes to interdigitation of the central C.G:G.C base pair step. Modeling studies, supported by genetic and biochemical data, show that the stereochemistry of the backbone atoms of the abutting hinge helices combined with the rigidity of the kinked base pair step constrain the interdigitating residue to leucine or methionine for the LacI/GalR family of transcription regulators.

About this Structure

1VPW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The structure of PurR mutant L54M shows an alternative route to DNA kinking., Arvidson DN, Lu F, Faber C, Zalkin H, Brennan RG, Nat Struct Biol. 1998 Jun;5(6):436-41. PMID:9628480 Page seeded by OCA on Sat May 3 12:46:40 2008

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